Library Open Repository

Practical method for evaluation of linearity and effective pathlength of on-capillary photometric detectors in capillary electrophoresis

Downloads

Downloads per month over past year

Johns, CA and Macka, M and Haddad, PR and King, M and Paull, B (2001) Practical method for evaluation of linearity and effective pathlength of on-capillary photometric detectors in capillary electrophoresis. Journal of Chromatography A, 927 (1-2). pp. 237-241. ISSN 0021-9673

[img] PDF
269.pdf.pdf | Request a copy
Full text restricted
Available under University of Tasmania Standard License.

Abstract

The optical characteristics of on-capillary photometric detectors for capillary electrophoresis were evaluated and five commercial detectors were compared. Plots of sensitivity (absorbance/concentration) versus absorbance obtained with a suitable testing solution yield both the linear range and the effective path length of the detector. The detector linearity is a crucial parameter when using absorbing electrolytes, such as for indirect photometric detection, and especially for highly absorbing electrolyte probe ions. The upper limits of the linear ranges (determined as 5% decline in sensitivity) for five commercial detectors ranged from 0.175 to 1.2 AU. The effective pathlength reflects the quality of the optical design of the detector and is equal to the capillary internal diameter only for a light beam passing exactly through the capillary centre, but becomes progressively shorter for imperfect optical designs. The determined effective pathlength for the five investigated detectors ranged from 49.7 to 64.6 μm for a 75 μm I.D. capillary.

Item Type: Article
Keywords: Detection, electrophoresis; Photometric detection; Indirect detection; Instrumentation
Journal or Publication Title: Journal of Chromatography A
Page Range: pp. 237-241
ISSN: 0021-9673
Identification Number - DOI: 10.1016/S0021-9673(01)01095-0
Additional Information: The definitive version is available at http://www.sciencedirect.com
Date Deposited: 25 Oct 2008 02:46
Last Modified: 18 Nov 2014 03:52
URI: http://eprints.utas.edu.au/id/eprint/7848
Item Statistics: View statistics for this item

Repository Staff Only (login required)

Item Control Page Item Control Page