Library Open Repository

Retention modelling of electrostatic and adsorption effects of aliphatic and aromatic carboxylic acids in ion-exclusion chromatography II. Calculations of adsorption coefficients in unbuffered eluents

Downloads

Downloads per month over past year

Ng, KL and Glod, BK and Dicinoski, GW and Haddad, PR (2001) Retention modelling of electrostatic and adsorption effects of aliphatic and aromatic carboxylic acids in ion-exclusion chromatography II. Calculations of adsorption coefficients in unbuffered eluents. Journal of Chromatography A, 920 (1-2). pp. 41-49. ISSN 0021-9673

[img] PDF
257.pdf.pdf | Request a copy
Full text restricted
Available under University of Tasmania Standard License.

Abstract

Previous models for the retention behaviour of carboxylic acids in ion-exclusion chromatography are applicable only when the degree of ionisation of the analyte is constant over the entire chromatographic peak. When solutions of sulfuric acid are used as eluents, this condition applies only when the eluent concentration is considerably higher than that of the analyte. Since it is common for dilute solutions of sulfuric acid to be used as eluents, a retention model which accounts for unbuffered eluents has been developed. This model also considers the effects on retention of hydrophobic adsorption of the undissociated and dissociated forms of the analyte onto the stationary phase substrate, as well as the effects of organic solvents added to the eluent. The derivation of this model is presented and it has been evaluated using a comprehensive set of retention data obtained using three different sulfonated stationary phases over a range of eluent conditions. The adsorption coefficients calculated from the model are in accordance with expected trends and showed that both the undissociated and dissociated forms of the analyte acids were retained by hydrophobic adsorption effects, although this adsorption was much stronger for the undissociated analytes.

Item Type: Article
Keywords: Carboxylic acids; Retention models; Mathematical modelling; Ion-exclusion chromatography; Adsorption
Journal or Publication Title: Journal of Chromatography A
Page Range: pp. 41-49
ISSN: 0021-9673
Identification Number - DOI: 10.1016/S0021-9673(01)00608-2
Additional Information: The definitive version is available at http://www.sciencedirect.com
Date Deposited: 25 Oct 2008 02:44
Last Modified: 18 Nov 2014 03:52
URI: http://eprints.utas.edu.au/id/eprint/7852
Item Statistics: View statistics for this item

Repository Staff Only (login required)

Item Control Page Item Control Page