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Indirect photometric detection in capillary electrophoresis using buffered electrolytes. Part I: principles

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Macka, M and Johns, CA and Doble, P and Haddad, PR (2001) Indirect photometric detection in capillary electrophoresis using buffered electrolytes. Part I: principles. L c G c North America: the magazine of separation science, 19 (1). pp. 38-47. ISSN 1527-5949

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Abstract

Indirect photometric
detection in capillary
electrophoresis is valuable
as a detection method for
nonabsorbing low
molecular weight ions.
The general operating
principle rests in the
displacement of an
absorbing ion —
sometimes called the
probe — added to the
electrolyte by the analyte
co-ions, which are ions of
the same charge polarity,
as they migrate under the
influence of the electric
field through the
capillary. Despite this
apparent simplicity, the
choice of optimal
parameters, and
especially of electrolyte
composition, can be quite
challenging. This first
installment of a two-part
series outlines the
principles and approaches
to indirect detection.

Item Type: Article
Journal or Publication Title: L c G c North America: the magazine of separation science
Page Range: pp. 38-47
ISSN: 1527-5949
Additional Information:

Copyright © 2001 Advanstar Communications Inc.

Date Deposited: 05 Dec 2008 03:26
Last Modified: 18 Nov 2014 03:52
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