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Indirect photometric detection in capillary electrophoresis using buffered electrolytes. Part I: principles

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Macka, M and Johns, CA and Doble, P and Haddad, PR (2001) Indirect photometric detection in capillary electrophoresis using buffered electrolytes. Part I: principles. L c G c North America: the magazine of separation science, 19 (1). pp. 38-47. ISSN 1527-5949

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Abstract

Indirect photometric detection in capillary electrophoresis is valuable as a detection method for nonabsorbing low molecular weight ions. The general operating principle rests in the displacement of an absorbing ion — sometimes called the probe — added to the electrolyte by the analyte co-ions, which are ions of the same charge polarity, as they migrate under the influence of the electric field through the capillary. Despite this apparent simplicity, the choice of optimal parameters, and especially of electrolyte composition, can be quite challenging. This first installment of a two-part series outlines the principles and approaches to indirect detection.

Item Type: Article
Journal or Publication Title: L c G c North America: the magazine of separation science
Page Range: pp. 38-47
ISSN: 1527-5949
Additional Information: Copyright © 2001 Advanstar Communications Inc.
Date Deposited: 05 Dec 2008 03:26
Last Modified: 18 Nov 2014 03:52
URI: http://eprints.utas.edu.au/id/eprint/7907
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