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Investigations on the behaviour of acidic, basic and neutral compounds in capillary electrochromatography on a mixed-mode stationary phase

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Klampfl, CW and Hilder, EF and Haddad, PR (2000) Investigations on the behaviour of acidic, basic and neutral compounds in capillary electrochromatography on a mixed-mode stationary phase. Journal of Chromatography A, 888 (1-2). pp. 267-274. ISSN 0021-9673

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Abstract

This work describes the separation of acidic, basic and neutral organic compounds as well as inorganic anions in a single run by capillary electrochromatography employing a stationary phase which exhibits both strong anion-exchange and reversed-phase chromatographic characteristics. The positive surface charge of this stationary phase provided a substantial anodic electroosmotic flow. The analytes were separated by a mixed-mode mechanism which comprised chromatographic interactions (hydrophobic interactions, ion-exchange) as well as electrophoretic migration. The influence of ion-exchange and hydrophobic interactions on the retention/migration of the analytes could be manipulated by varying the concentration of a competing ion and/or the amount of organic modifier present in the background electrolyte. Additionally the effects of pH changes on both the chromatographic interactions as well as the electrophoretic migration of the analytes were investigated.

Item Type: Article
Keywords: Electrochromatography; Mixed-mode retention; Anion exchange; Stationary phases, CEC
Journal or Publication Title: Journal of Chromatography A
Page Range: pp. 267-274
ISSN: 0021-9673
Identification Number - DOI: 10.1016/S0021-9673(00)00613-0
Additional Information: The definitive version is available at http://www.sciencedirect.com
Date Deposited: 31 Oct 2008 05:52
Last Modified: 18 Nov 2014 03:52
URI: http://eprints.utas.edu.au/id/eprint/7920
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