Library Open Repository

Design of background electrolytes for indirect detection of anions by capillary electrophoresis

Downloads

Downloads per month over past year

Doble, P and Macka, M and Haddad, PR (2000) Design of background electrolytes for indirect detection of anions by capillary electrophoresis. TrAC - Trends in Analytical Chemistry, 19 (1). pp. 10-17. ISSN 0167-2940

[img] PDF
217.pdf.pdf | Request a copy
Full text restricted
Available under University of Tasmania Standard License.

Abstract

In capillary electrophoresis of inorganic and other low molecular weight anions using indirect photometric detection, the correct design of the background electrolyte can considerably reduce the time needed for method development and can increase the quality of the separation achieved. This article discusses the basic steps for development of suitable electrolytes, based on fundamental knowledge of the nature of the analytes and the proposed background electrolyte. Consideration is given to the choice of the absorbing probe used for indirect detection, based on the relative mobilities of the probe and the analytes, and the desired limits of detection. Choice of the electro-osmotic flow modifier and correct buffering protocols are also discussed. Finally, strategies to increase the useful mobility range of the background electrolyte are given, based on matching the mobilities of multiple electrolyte probes with the mobilities of the analytes.

Item Type: Article
Keywords: Indirect detection; Background electrolyte; Photometric detection, indirect
Journal or Publication Title: TrAC - Trends in Analytical Chemistry
Page Range: pp. 10-17
ISSN: 0167-2940
Identification Number - DOI: 10.1016/S0165-9936(99)00181-8
Additional Information: The definitive version is available at http://www.sciencedirect.com
Date Deposited: 31 Oct 2008 06:11
Last Modified: 18 Nov 2014 03:52
URI: http://eprints.utas.edu.au/id/eprint/7931
Item Statistics: View statistics for this item

Repository Staff Only (login required)

Item Control Page Item Control Page