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Retention modelling of electrostatic and adsorption effects of aliphatic and aromatic carboxylic acids in ion-exclusion chromatography

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Ng, KL and Paull, B and Haddad, PR and Tanaka, K (1999) Retention modelling of electrostatic and adsorption effects of aliphatic and aromatic carboxylic acids in ion-exclusion chromatography. Journal of Chromatography A, 850 (1-2). pp. 17-27. ISSN 0021-9673

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Abstract

The retention mechanism of aliphatic and aromatic carboxylic acids in ion-exclusion chromatography has been investigated with consideration of simultaneous electrostatic repulsion effects and hydrophobic adsorption effects. A mathematical relationship between the retention factor of the analyte and the mobile-phase composition (sulfuric acid concentration and percentage of methanol), the type of analyte (pKa and hydrophobicity) and some physical characteristics of the stationary phase has been derived. Thirteen carboxylic acids (comprising mono- and divalent, aliphatic and aromatic acids) were chosen and used to acquire retention data on three different cation-exchange stationary phases (in which the sulfonate functional groups are bound to polystyrene–divinylbenzene, polymethacrylate or silica) using 14 mobile-phase compositions of varying pH and percent methanol. These retention data were used to derive the parameters necessary to solve the retention model using non-linear regression. In this way, a quantitative measure of the effects of adsorption phenomena on analyte retention were obtained. The model was then used to optimise the separation of nine carboxylic acids.

Item Type: Article
Keywords: Carboxylic acids; Retention models; Optimization; Ion-exclusion chromatography
Journal or Publication Title: Journal of Chromatography A
Page Range: pp. 17-27
ISSN: 0021-9673
Identification Number - DOI: 10.1016/S0021-9673(99)00735-9
Additional Information: The definitive version is available at http://www.sciencedirect.com
Date Deposited: 31 Oct 2008 06:33
Last Modified: 18 Nov 2014 03:53
URI: http://eprints.utas.edu.au/id/eprint/7938
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