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Mitogen-induced responses in lymphocytes from platypus, the Tasmanian devil and the eastern barred bandicoot
Stewart, NJ and Bettiol, SS and Kreiss, A and Fox, N and Woods, GM (2008) Mitogen-induced responses in lymphocytes from platypus, the Tasmanian devil and the eastern barred bandicoot. Australian Veterinary Journal, 86 (10). pp. 408-413.
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OBJECTIVE: As the platypus (Ornithorhynchus anatinus), the Tasmanian devil (Sarcophilus harrisi) and the eastern barred bandicoot (Perameles gunni) are currently at risk of serious population decline or extinction from fatal diseases in Tasmania, the goal of the present study was to describe the normal immune response of these species to challenge using the lymphocyte proliferation assay, to give a solid basis for further studies.
METHODS: For this preliminary study, we performed lymphocyte proliferation assays on peripheral blood mononuclear cells (PBMC) from the three species. We used the common mitogens phytohaemagglutinin (PHA), concanavalin A (ConA), lipopolysaccharide (LPS) and pokeweed mitogen (PWM).
RESULTS: All three species recorded the highest stimulation index (SI) with the T-cell mitogens PHA and ConA. Tasmanian devils and bandicoots had greater responses than platypuses, although variability between individual animals was high.
CONCLUSION: For the first time, we report the normal cellular response of the platypus, the Tasmanian devil and the eastern barred bandicoot to a range of commonly used mitogens.
|Keywords:||Australia • bandicoot • immunology • lymphocyte proliferation assay • mycology • platypus • Tasmanian devil • wildlife|
|Journal or Publication Title:||Australian Veterinary Journal|
|Page Range:||pp. 408-413|
|Identification Number - DOI:||10.1111/j.1751-0813.2008.00349.x|
The definitive published version is available online at: http://interscience.wiley.com
|Date Deposited:||14 Dec 2008 22:11|
|Last Modified:||18 Nov 2014 03:54|
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