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Micelle to solvent stacking of organic cations in capillary zone electrophoresis with electrospray ionization mass spectrometry

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Quirino, JP (2009) Micelle to solvent stacking of organic cations in capillary zone electrophoresis with electrospray ionization mass spectrometry. Journal of Chropmatography A, 1216 (2). pp. 294-299. ISSN 0021-9673

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Abstract

The direction of the effective electrophoretic mobility of small organic cations in micellar electrokinetic chromatography using sodium dodecyl sulphate in a low-pH electrolyte can be reversed in the presence of organic solvent. This effective electrophoretic mobility change is presented here as a new dimension for on-line sample preconcentration of cations in capillary zone electrophoresis (CZE) using a background solution (BGS) modified by an organic solvent. The sample is prepared in a micellar solution without organic solvent. The focusing effect relies on the reversal in the effective electrophoretic mobility at the boundary zone between the micellar matrix and the BGS modified with organic solvent. This on-line sample preconcentration technique, called micelle to solvent stacking (MSS) afforded more than an order of magnitude improvement in concentration sensitivity compared to typical CZE-UV or CZE-electrospray ionization (ESI) MS analysis. The calculated limit of detection (S/N = 3) for pindolol and metoprolol analysed by MSS-CZE-ESI-MS was found to be 0.03 and 0.01 μg/mL, respectively.

Item Type: Article
Keywords: On-line sample preconcentration; Capillary zone electrophoresis; Electrospray ionization mass spectrometry; Adrenergic β-blockers; Micelle to solvent stacking
Journal or Publication Title: Journal of Chropmatography A
Page Range: pp. 294-299
ISSN: 0021-9673
Identification Number - DOI: 10.1016/j.chroma.2008.11.062
Additional Information: The definitive version is available at http://www.sciencedirect.com
Date Deposited: 18 Jan 2009 22:22
Last Modified: 18 Nov 2014 03:54
URI: http://eprints.utas.edu.au/id/eprint/8226
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