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Effect of extended-spectrum beta-lactamases on the susceptibility of Haemophilus influenzae to cephalosporins

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Tristram, SG (2003) Effect of extended-spectrum beta-lactamases on the susceptibility of Haemophilus influenzae to cephalosporins. Journal of Antimicrobial Chemotherapy, 51. pp. 39-43. ISSN 0305-7453

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Abstract

The extended-spectrum beta-lactamases (ESBLs) TEM-3, TEM-4 and TEM-5 were cloned into Haemophilus influenzae. These recombinant strains exhibited cefotaxime MICs of 0.5, 0.25 and 0.12 mg/L for TEM-3, -4 and -5, respectively, and the MIC of cefaclor was 4.0 mg/L. These MICs are higher than those of beta-lactamase-negative strains, or those producing simple wild-type TEM-1 beta-lactamase, but not high enough to be categorized as resistant according to the breakpoints of the NCCLS. The clones were also categorized as susceptible using NCCLS disc diffusion methodology and interpretive criteria. This study shows that current NCCLS susceptibility testing methods may have difficulty in detecting ESBLs if they were to occur in H. influenzae.

Item Type: Article
Keywords: extended-spectrum beta-lactamase, TEM beta-lactamase
Journal or Publication Title: Journal of Antimicrobial Chemotherapy
Page Range: pp. 39-43
ISSN: 0305-7453
Identification Number - DOI: 10.1093/jac/dkg015
Additional Information: This is a pre-copy-editing, author-produced PDF of an article accepted for publication in Journal of Antimicrobial Chemotherapy following peer review. The definitive publisher-authenticated version Tristram, Stephen G (2003) Effect of extended-spectrum beta-lactamases on the susceptibility of Haemophilus influenzae to cephalosporins. Journal of Antimicrobial Chemotherapy, 51. pp. 39-43 is available online at:http://jac.oxfordjournals.org/
Date Deposited: 16 Mar 2007
Last Modified: 18 Nov 2014 03:13
URI: http://eprints.utas.edu.au/id/eprint/852
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