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Determination of protein synthesis in rainbow trout, Oncorhynchus mykiss, using a stable isotope
Carter, CG and Owen, SF and He, ZY and Watt, PW and Scrimgeour, C and Houlihan, DF and Rennie, MJ (1994) Determination of protein synthesis in rainbow trout, Oncorhynchus mykiss, using a stable isotope. Journal of Experimental Biology, 189. pp. 279-284.
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It has been suggested (Houlihan, 1991) that the consumption of 1 g of protein in a variety of species of fish stimulates the synthesis of, approximately, an equal amount of protein. Although synthesis of protein may account for as much as 40 % of the wholeanimal oxygen consumption (Lyndon et al. 1992), only about 30 % of the synthesized proteins are retained as growth (Houlihan et al. 1988; Carter et al. 1993a,b). Thus, one focus of attention is the potential advantage gained by fish in allocating a considerable proportion of assimilated energy to protein turnover in contrast to relatively low-cost, low-turnover protein growth (Houlihan et al. 1993). Rates of protein synthesis in several species of fish have been measured using radioactively labelled amino acids, frequently given as a flooding dose (reviewed by Fauconneau, 1985; Houlihan, 1991). These measurements cannot be made for longer than a few hours because of the decline in specific radioactivity in the amino acid free pool. However, as protein synthesis rates vary during the course of a day as a result of the post-prandial stimulation, and since radiolabelled amino acid methodology is invasive, short-term and terminal, it has been difficult to be certain of the relationship between protein growth measured in the long term and protein synthesis rates measured in the short term. This paper addresses these problems by developing a method using 15N in orally administered protein to measure protein synthesis rates in fish over relatively long periods, the aim being to use procedures that are as non-invasive and repeatable as possible.
|Journal or Publication Title:||Journal of Experimental Biology|
|Page Range:||pp. 279-284|
|Date Deposited:||23 Mar 2007|
|Last Modified:||18 Nov 2014 03:13|
|Item Statistics:||View statistics for this item|
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