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A rapid flow cytometric technique for the detection of platelet-monocyte complexes, activated platelets and platelet-derived microparticles
Pearson, L and Thom, J and Adams, MJ and Oostryck, R and Krueger, R and Yong, G and Baker, RI (2009) A rapid flow cytometric technique for the detection of platelet-monocyte complexes, activated platelets and platelet-derived microparticles. International Journal of Laboratory Hematology, 31 (4). p. 430. ISSN 1751-5521
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Platelet activation occurs in a variety of clinical situations in which it directly contributes to the pathology. This study reports a simple flow cytometric assay for platelet activation which measures platelet-derived microparticles, activated platelets and platelet–monocyte complexes. Pre- and post analytical conditions were investigated and optimized and a normal range established on 20 healthy controls. Twenty patients pre- and post percutaneous coronary intervention (PCI) were tested with the technique. Soluble activation markers sCD40 ligand and sPselectin and plasma phospholipid levels were measured in both groups. There was a significant increase in activated platelets and platelet– monocyte complexes between normal and pre-PCI (P = 0.005 and 0.0275, respectively) suggesting an activated state. There was a significant fall in activated platelets post-PCI (P = 0.0027) which was mirrored by a fall in soluble CD40 ligand, soluble P-selectin and plasma phospholipid levels (P = 0.0066, <0.0001 and 0.0032, respectively) consistent with antiplatelet therapy administered during the process. This is a reliable and rapid method for the assessment of ex vivo platelet activation which may be an aid in diagnosis and help guide therapy for patients with thrombotic disease.
|Keywords:||Activated platelets, flow cytometry, percutaneous coronary intervention, platelet–monocyte complexes|
|Journal or Publication Title:||International Journal of Laboratory Hematology|
|Page Range:||p. 430|
|Identification Number - DOI:||10.1111/j.1751-553X.2008.01059.x|
|Additional Information:||The original publication is available at http://www.interscience.wiley.com/|
|Date Deposited:||27 Jul 2010 07:05|
|Last Modified:||18 Nov 2014 04:12|
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