A rapid flow cytometric technique for the detection of platelet-monocyte complexes, activated platelets and platelet-derived microparticles
Pearson, L and Thom, J and Adams, MJ and Oostryck, R and Krueger, R and Yong, G and Baker, RI (2009) A rapid flow cytometric technique for the detection of platelet-monocyte complexes, activated platelets and platelet-derived microparticles. International Journal of Laboratory Hematology, 31 (4). 430 . ISSN 1751-5521
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Official URL: http://dx.doi.org/10.1111/j.1751-553X.2008.01059.x
Platelet activation occurs in a variety of clinical situations in which it
directly contributes to the pathology. This study reports a simple flow
cytometric assay for platelet activation which measures platelet-derived
microparticles, activated platelets and platelet–monocyte complexes.
Pre- and post analytical conditions were investigated and optimized
and a normal range established on 20 healthy controls. Twenty patients
pre- and post percutaneous coronary intervention (PCI) were tested
with the technique. Soluble activation markers sCD40 ligand and sPselectin
and plasma phospholipid levels were measured in both groups.
There was a significant increase in activated platelets and platelet–
monocyte complexes between normal and pre-PCI (P = 0.005 and
0.0275, respectively) suggesting an activated state. There was a
significant fall in activated platelets post-PCI (P = 0.0027) which was
mirrored by a fall in soluble CD40 ligand, soluble P-selectin and plasma
phospholipid levels (P = 0.0066, <0.0001 and 0.0032, respectively)
consistent with antiplatelet therapy administered during the process.
This is a reliable and rapid method for the assessment of ex vivo platelet
activation which may be an aid in diagnosis and help guide therapy for
patients with thrombotic disease.
|Additional Information:||The original publication is available at
|Keywords:||Activated platelets, flow cytometry,
percutaneous coronary intervention,
|Deposited By:||Dr Murray J Adams|
|Deposited On:||27 Jul 2010 17:05|
|Last Modified:||27 Aug 2013 09:35|
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