title: Oxidative stress and lipid-derived inflammatory mediators during acute exacerbations of cystic fibrosis
creator: Reid, DW
creator: Misso, N
creator: Aggarwal, S
creator: Thompson, PJ
creator: Walters, EH
subject: 321027 Respiratory Diseases
description: Oxidative stress and lipid-derived inflammatory mediators during acute exacerbations of cystic
fibrosis
REID DW, MISSO N, AGGARWAL S, THOMPSON PJ, WALTERS EH. Respirology 2007; 12: 63–69
Background and objective: In cystic fibrosis (CF) very few studies have assessed sputum 8-iso-
PGF2α levels during pulmonary exacerbations as a direct measure of airway oxidative stress. The role
of other lipid-derived inflammatory mediators, such as the cysteinyl leukotrienes (cys-LTs) and prostaglandin
(PG)-E2, during exacerbations is also poorly defined and the effect of conventional antibiotic
therapy on these components of the inflammatory process is unclear.
Methods: Sputum 8-iso-PGF2α, total cys-LT and PGE2 levels were measured in 17 CF patients experiencing
a pulmonary exacerbation and repeated analysis were performed in 15 of these patients
after antibiotic treatment. Eight stable CF and nine healthy subjects provided control data.
Results: Sputum 8-iso-PGF2α was significantly elevated in acute, but not stable CF patients versus
healthy controls (P < 0.001). Similarly, sputum cys-LT and PGE2 levels were increased in acute compared
with stable CF patients and healthy controls (P ≤ 0.001). Although substantially lower than in
acute patients, sputum cys-LT levels in stable patients were also significantly higher than in normal
controls (P = 0.01). There were strong associations between cys-LT levels and sputum total cell
counts, and blood neutrophils in acute patients (r2 = 0.53, P = 0.001 and r2 = 0.33, P < 0.05, respectively).
Overall in the CF patients, FEV1%predicted was strongly and negatively correlated with sputum
8-iso-PGF2α (r2 = 0.34, P = 0.006), cys-LT (r2 = 0.40, P = 0.002) and PGE2 (r2 = 0.52, P < 0.001) levels.
Antibiotic treatment reduced sputum total cell count (P = 0.03), but did not affect 8-iso-PGF2α, cys-LT
or PGE2 levels.
Conclusions: CF exacerbations are characterized by increased oxidative stress and sputum concentrations
of bioactive lipid mediators. Treatment does not modulate these aspects of inflammation
and more targeted therapy needs further study.
publisher: Wiley-Blackwell Publishing Asia
date: 2007
type: Article
type: PeerReviewed
format: application/pdf
identifier: http://eprints.utas.edu.au/3971/1/3971.pdf
relation: http://dx.doi.org/10.1111/j.1440-1843.2006.00962.x
identifier: Reid, DW and Misso, N and Aggarwal, S and Thompson, PJ and Walters, EH (2007) Oxidative stress and lipid-derived inflammatory mediators during acute exacerbations of cystic fibrosis. Respirology, 12 (1). pp. 63-69. ISSN 1323-7799
relation: http://eprints.utas.edu.au/3971/