6424 35 archive 310 disk0/00/00/64/24 2008-05-19 12:31:41 2008-07-18 10:58:04 2008-07-16 17:24:14 article show Paul.Haddad@utas.edu.au Murrihy JP Breadmore MC mcb@utas.edu.au Tan A McEnery M Alderman J O'Mathuna C O'Neill AP O'Brien P Avdalovic N Haddad PR Paul.Haddad@utas.edu.au Glennon JD Ion chromatography on-chip pub 030108 250000 250401 250400 970103 780103 restricted Inorganic anions; Thiourea; Chip technology; Instrumentation; Latex nanoparticles; Microseparation The definitive version is available online at http://www.sciencedirect.com/ On-chip separation of inorganic anions by ion-exchange chromatography was realized. Micro separation channels were fabricated on a silicon wafer and sealed with a Pyrex cover plate using standard photolithography, wet and dry chemical etching, and anodic bonding techniques. Quaternary ammonium latex particles were employed for the first time to coat the separation channels on-chip. Owing to the narrow depths of the channels on the chip, 0.5–10 μm, there were more interactions of the analytes with the stationary phase on the chip than in a 50-μm I.D. capillary. With off-chip injection (20 nl) and UV detection, NO2−, NO3−, I−, and thiourea were separated using 1 mM KCl as the eluent. The linear ranges for NO2− and NO3− are from 5 to 1000 μM with the detection limits of 0.5 μM. 2001-07-27 published Journal of Chromatography A 924 1-2 233-238 10.1016/S0021-9673(01)00855-X TRUE 0021-9673 http://dx.doi.org/10.1016/S0021-9673(01)00855-X 1. C.S. Effenhauser Topics Curr. Chem. 194 (1998), p. 51. 2. D. Figeys and D. Pinto Anal. Chem. 72 (2000), p. 330A. 3. F.E. Regnier, B. He, S. Lin and J. Busse Trends Biotechnol. 17 (1999), p. 101. 4. G. Ocvirk, E. Verpoorte, A. Manz, M. Grasserbauer and H.M. Widmer Anal. Methods Instrum. 2 2 (1995), p. 74. 5. M. McEnery, J.D. Glennon, J. Alderman and S.C. O’Mathuna J. Cap. Electrophoresis Microchip Technol. 6 (1999), p. 33. 6. C. Ericson, J. Holm, T. Ericson and S. Hjerten Anal. Chem. 72 (2000), p. 81. 7. B. He, N. Tait and F. Regnier Anal. Chem. 70 (1998), p. 3790. 8. M.C. Breadmore, M. Macka, N. Avdalovic and P.R. Haddad Analyst 125 (2000), p. 1235. 9. M.C. Bruzzoniti, E. Mentasti and C. Sarzanini Anal. Chim. Acta 382 (1999), p. 291. 10. D.J. Pyo, P.K. Dasgupta and L.S. Yengoyan Anal. Sci. 13 (1997), p. 185. 11. M. McEnery, A. Tan, J. Alderman, J. Patterson, C. O’Mathuna and J.D. Glennon Analyst 125 (2000), p. 25. 5278 6 6424 1 application/pdf en staffonly cc_utas
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