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Construction of a high-density composite map and comparative mapping of segregation distortion regions (SDRs) in barley.

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Li, H and Kilian, A and Zhou, M and Wenzl, P and Huttner, E and Mendham, NJ and McIntyre, L and Vaillancourt, RE (2010) Construction of a high-density composite map and comparative mapping of segregation distortion regions (SDRs) in barley. Molecular Genetics and Genomics, 284 (5). pp. 319-331. ISSN 1617-4615

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Abstract

Segregation distortion can negatively impact on
gains expected using selection. In order to increase our
understanding of genetic factors that may influence the
extent and direction of segregation distortion, segregation
distortion analyses were conducted in four different doubled
haploid (DH) populations. A high-density composite
map of barley was then constructed by integrating information from the four populations. The composite map
contained 2,111 unique loci, comprising RFLP, SSR and
DArT markers and spanned 1,136 cM. In the four populations
investigated, the proportion of markers with
segregation distortion ranged from 15 to 38%, depending
on the population. The highest distortion was observed in
populations derived by the microspore culture technique.
Distorted loci tended to be clustered, which allowed definition of segregation distortion regions (SDRs). A total of 14 SDRs were identified in the 4 populations. Using the high-density composite map, several SDRs were shown tohave consistent map locations in two or more populations; one SDR on chromosome 1H was present in all four populations. The analysis of haplotypes underlying seven SDRs indicated that in three cases the under-represented haplotypes were common across populations, but for four SDRs the under-represented haplotypes varied across
populations. Six of the seven centromeric regions harboured
SDRs suggesting that genetic processes related to
position near a centromere caused the segregation distortion
in these SDRs. Other SDRs were most likely due to the
methods used to produce the DH populations. The association
of the SDRs identified in this study and some of the
genes involved in the process of haploid production
described in other studies were compared. The composite
map constructed in this study provides an additional
resource for the barley community via increased genome
coverage and the provision of additional marker options. It
has also enabled further insights into mechanisms that
underpin segregation distortion.

Item Type: Article
Keywords: Barley Composite map Segregation distortion regions Comparative mapping Haplotypes
Journal or Publication Title: Molecular Genetics and Genomics
Page Range: pp. 319-331
ISSN: 1617-4615
Identification Number - DOI: 10.1007/s00438-010-0570-3
Additional Information:

The original publication is available at www.springerlink.com

Date Deposited: 29 Nov 2010 05:42
Last Modified: 18 Nov 2014 04:14
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