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Manufacturing and application of a fully polymeric electrophoresis chip with integrated polyaniline electrodes

Henderson, RD, Guijt, RM, Haddad, PR, Hilder, EF, Lewis, T and Breadmore, MC 2010 , 'Manufacturing and application of a fully polymeric electrophoresis chip with integrated polyaniline electrodes' , Lab on a Chip, vol. 10, no. 14 , pp. 1869-1872 , doi:

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This work describes the development of a fully polymeric microchip with integrated polymeric
electrodes suitable for performing microchip electrophoresis. The polymer electrodes were fabricated in
a thin film of the conducting polymer, polyaniline (PANI), by flash lithography using a studio camera
flash and a transparency mask. During flash welding, exposed regions welded into non-conducting
regions forming a conducting polymer circuit in the non-exposed regions. Using a structured layer of
dry film photoresist for sealing, a polydimethylsiloxane (PDMS) substrate containing channels and
reservoirs was bound to the PANI film to form an integrated microfluidic device. The conducting
regions of the PANI film were shown to be capable of carrying the high voltages of up to 2000 V
required for chip electrophoresis, and were stable for up to 30 minutes under these conditions. The
PANI electrodes were used for the electrophoretic separation of three sugars labelled with 8-amino-
1,3,6-pyrenetrisulfonic acid (APTS) in the dry film resist—PDMS hybrid device. Highly efficient
separations comparable to those achieved in similar microchips using platinum electrodes confirm the
potential of polyaniline as a new material suitable for high voltage electrodes in Lab-on-a-chip devices.

Item Type: Article
Authors/Creators:Henderson, RD and Guijt, RM and Haddad, PR and Hilder, EF and Lewis, T and Breadmore, MC
Journal or Publication Title: Lab on a Chip
ISSN: 1473-0197
DOI / ID Number:
Additional Information:

Copyright © 2010 The Royal Society of Chemistry

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