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Gene expression profiling identifies two regulatory genes controlling dormancy and ABA sensitivity in Arabidopsis seeds

Version 2 2023-06-23, 11:04
Version 1 2023-05-26, 10:27
journal contribution
posted on 2023-06-23, 11:04 authored by JM Barrero, AA Millar, J Griffiths, T Czechowski, WR Scheible, M Udvardi, James ReidJames Reid, John RossJohn Ross, JV Jacobsen, F Gubler
Seed dormancy is a very important trait that maximizes the survival of seed in nature, the control of which can have important repercussions on the yield of many crop species. We have used gene expression profiling to identify genes that are involved in dormancy regulation in Arabidopsis thaliana. RNA was isolated from imbibed dormant (D) and after-ripened (AR) ecotype C24 seeds, and then screened by quantitative RT-PCR (qRT-PCR) for differentially expressed transcription factors (TFs) and other regulatory genes. Out of 2207 genes screened, we have identified 39 that were differentially expressed during the first few hours of imbibition. After analyzing T-DNA insertion mutants for 22 of these genes, two displayed altered dormancy compared with the wild type. These mutants are affected in genes that encode a RING finger and an HDZip protein. The first, named DESPIERTO, is involved in ABA sensitivity during seed development, regulates the expression of ABI3, and produces a complete loss of dormancy when mutated. The second, the HDZip (ATHB20), is expressed during seed germination in the micropylar endosperm and in the root cap, and increases ABA sensitivity and seed dormancy when mutated.

History

Publication title

the Plant Journal

Volume

61

Issue

4

Pagination

611-622

ISSN

0032-0935

Department/School

Biological Sciences

Publisher

Blackwell Publishing Ltd

Publication status

  • Published

Rights statement

The definitive published version is available online at: http://onlinelibrary.wiley.com

Repository Status

  • Restricted

Socio-economic Objectives

260599 Horticultural crops not elsewhere classified

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