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Gene expression profiling identifies two regulatory genes controlling dormancy and ABA sensitivity in Arabidopsis seeds


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Barrero, JM, Millar, AA, Griffiths, J, Czechowski, T, Scheible, WR, Udvardi, M, Reid, JB, Ross, JJ, Jacobsen, JV and Gubler, F 2010 , 'Gene expression profiling identifies two regulatory genes controlling dormancy and ABA sensitivity in Arabidopsis seeds' , the Plant Journal, vol. 61 , pp. 611-621 , doi: 10.1111/j.1365-313X.2009.04088.x.

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Seed dormancy is a very important trait that maximizes the survival of seed in nature, the control of which can
have important repercussions on the yield of many crop species. We have used gene expression profiling to
identify genes that are involved in dormancy regulation in Arabidopsis thaliana. RNA was isolated from
imbibed dormant (D) and after-ripened (AR) ecotype C24 seeds, and then screened by quantitative RT-PCR
(qRT-PCR) for differentially expressed transcription factors (TFs) and other regulatory genes. Out of 2207 genes
screened, we have identified 39 that were differentially expressed during the first few hours of imbibition. After
analyzing T-DNA insertion mutants for 22 of these genes, two displayed altered dormancy compared with the
wild type. These mutants are affected in genes that encode a RING finger and an HDZip protein. The first,
named DESPIERTO, is involved in ABA sensitivity during seed development, regulates the expression of ABI3,
and produces a complete loss of dormancy when mutated. The second, the HDZip (ATHB20), is expressed
during seed germination in the micropylar

Item Type: Article
Authors/Creators:Barrero, JM and Millar, AA and Griffiths, J and Czechowski, T and Scheible, WR and Udvardi, M and Reid, JB and Ross, JJ and Jacobsen, JV and Gubler, F
Keywords: Arabidopsis seed hormone ABA abscisic acid
Journal or Publication Title: the Plant Journal
ISSN: 0032-0935
DOI / ID Number: 10.1111/j.1365-313X.2009.04088.x
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