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Brassinosteroids, de-etiolation and the re-emerging art of plant hormone quantification


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Symons, GM and Reid, JB 2008 , 'Brassinosteroids, de-etiolation and the re-emerging art of plant hormone quantification' , Plant Signaling & Behavior, vol. 3, no. 10 , pp. 868-870 , doi: 10.4161/psb.3.10.6063.

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An increase in the use of molecular techniques has provided a
significant insight into the function of genes, and how they are
regulated and interact. However, in the field of plant hormone
physiology, the increased use of these techniques has been accompanied
by a reduction in the direct measurement of plant hormone
levels by physiochemical methods. Instead, the transcript (mRNA)
levels of genes involved in hormone metabolism are often used to
predict endogenous hormone levels. The validity of this approach
was recently tested by comparing the expression of a range of genes
involved in BR synthesis, catabolism and perception, with the
actual endogenous BR levels in pea seedlings grown under different
light conditions.1,2 Based on this comparison, we now argue that
gene expression analysis alone is not always a reliable indicator of
endogenous hormone levels.

Item Type: Article
Authors/Creators:Symons, GM and Reid, JB
Keywords: brassinosteroid, gene expression, hormone levels, de-etiolation
Journal or Publication Title: Plant Signaling & Behavior
ISSN: 1559-2316
DOI / ID Number: 10.4161/psb.3.10.6063
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Copyright © 2008 Landes Bioscience

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