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Stems of the Arabidopsis pin1-1 mutant are not deficient in free indole-3-acetic acid


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Jones, SE, DeMeo, JS, Davies, NW, Noonan, SE and Ross, JJ 2005 , 'Stems of the Arabidopsis pin1-1 mutant are not deficient in free indole-3-acetic acid' , Planta, vol. 222 , pp. 530-534 , doi: 10.1007/s00425-005-1561-1.

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The pin1-1 mutant of Arabidopsis thaliana has
been pivotal for studies on auxin transport and on the
role of auxin in plant development. It was reported
previously that when whole shoots were analysed, levels
of the major auxin, indole-3-acetic acid (IAA) were
dramatically reduced in the mutant, compared with the
WT (Okada et al. 1991). The cloning of PIN1, however,
provided evidence that this gene encodes a facilitator of
auxin efflux, raising the question of how the pin1-1
mutation might reduce overall IAA levels as well as IAA
transport. We therefore re-examined IAA levels in
individual parts of pin1-1 and WT plants, focusing on
inflorescence stems. Our data show that there is in fact
no systemic IAA deficiency in the mutant. The previously
reported difference between mutant and WT may
have been due to the inclusion of reproductive structures
in the WT harvest: we show here that the inflorescence
itself contains high levels of IAA. We reconcile the
normal IAA levels of pin1-1 inflorescence stems with
their (previously-reported) reduced ability to transport
IAA by presenting evidence that the auxin in mutant
stems is not imported from their apical portion. Our
data also indicate that levels of another auxin, indole-3-
butyric acid (IBA), are very low in stems of the genotypes
used in this study.

Item Type: Article
Authors/Creators:Jones, SE and DeMeo, JS and Davies, NW and Noonan, SE and Ross, JJ
Journal or Publication Title: Planta
ISSN: 0032-0935
DOI / ID Number: 10.1007/s00425-005-1561-1
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