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Abstract

The pea (Pisum sativum) homolog, PsKO1, of the Arabidopsis GA3 gene was isolated. It codes for a cytochrome P450 from
the CYP701A subfamily and has ent-kaurene oxidase (KO) activity, catalyzing the three step oxidation of ent-kaurene to
ent-kaurenoic acid in the gibberellin (GA) biosynthetic pathway when expressed in yeast (Saccharomyces cerevisiae). PsKO1
is encoded by the LH gene because in three independent mutant alleles, lh-1, lh-2, and lh-3, PsKO1 has altered sequence, and
the lh-1 allele, when expressed in yeast, failed to metabolize ent-kaurene. The lh mutants of pea are GA deficient and have
reduced internode elongation and root growth. One mutant (lh-2) also causes a large increase in seed abortion. PsKO1 (LH)
is expressed in all tissues examined, including stems, roots, and seeds, and appears to be a single-copy gene. Differences in
sensitivity to the GA synthesis inhibitor, paclobutrazol, between the mutants appear to result from the distinct nature of the
genetic lesions. These differences may also explain the tissue-specific differences between the mutants.

Item Type:	Article
Authors/Creators:	Davidson, SE and Smith, JJ and Helliwell, CA and Poole, AT and Reid, JB
Journal or Publication Title:	Plant Physiology
ISSN:	0032-0889
DOI / ID Number:	​10.​1104/​pp.​103.​032706
Additional Information:	Copyright © 2004 American Society of Plant Biologists
Item Statistics:	View statistics for this item

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