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Abstract

The Le gene of pea encodes a gibberellin 3β-hydroxylase. Heterologous expression of the le-2 allele indicated that a truncated protein was produced, confirming that le-2 is a null mutation. The Le expression product was unable to metabolise GA12, but was able to produce a small quantity of GA8 from GA29. The le-2 mutation had no effect on the levels of GA1, GA4 or GA8 in developing seeds. Measurements of mRNA levels indicate that the Le gene is only weakly expressed in young pea seeds. These results explain why mutant alleles at the Le locus have no major impact on seed development, even though 3β-hydroxylated GAs are essential for normal seed development in pea. Rather, a second 3β-hydroxylase, with a different substrate specificity, may be expressed in young seeds, resulting in a different biosynthetic pathway leading to the biologically active GAs.

Item Type:	Article
Authors/Creators:	Lester, DR and MacKenzie-Hose, AK and Davies, PJ and Ross, JJ and Reid, JB
Journal or Publication Title:	Plant Growth Regulation
ISSN:	0167-6903
DOI / ID Number:	10.1023/A:1006158701016
Additional Information:	Copyright 1999 Kluwer Academic Publishers
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