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Biochemical composition during growth and starvation of early larval stages of cultured spiny lobster (Jasus edwardsii) phyllosoma


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Ritar, AJ, Dunstan, GA, Crear, BJ and Brown, MR 2003 , 'Biochemical composition during growth and starvation of early larval stages of cultured spiny lobster (Jasus edwardsii) phyllosoma' , Comparative Biochemistry and Physiology Part A, vol. 136, no. 2 , pp. 353-370 , doi: 10.1016/S1095-6433(03)00167-3.

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We examined biochemical changes accompanying feeding and starvation from hatch to Stage VI (day 74 after hatch) in spiny lobster, Jasus edwardsii, phyllosoma larvae. Larval dry weights (dw) increased 17-fold from hatch (80 +/- 1 ug) to Stage VI (1415 +/- 44 ug). Larvae starved for 6-11 days at Stages II, IV and VI were 14-40% lighter than their fed counterparts fed enriched Artemia. The increases and losses in total dw during feeding and starvation were associated with changes in the content of protein (constituting 31.4-41.7% of dw) and carbohydrate (constituting 2.6-5.3% of dw), while larger changes in lipid content indicated its greater importance as an energy substrate. Lipid content increased from 7.9% of dw at hatch to its highest of 12.5% at Stage IV, but declined by 50% or more during starvation. This suggests that protein, carbohydrate and lipid are all important energy stores, although lipids are catabolised at a greater rate during food deprivation. The principal lipid class was polar lipid (79-92% of total lipid), followed by sterol (6-20%), with triacylglycerol and other lipid classes at <2%. Polar lipids were catabolized and sterols were conserved during starvation. Changes in the fatty acid profile had mostly occurred before the first moult at day 8 after hatch, with gradual changes thereafter to Stage VI, reflecting their abundance in the Artemia diet. There was some conservation of the major essential fatty acids (EFA), 20:4n-6, 20:5n-3, 22:6n-3, and the fatty acid profile showed large gains in the C18 PUFA, 18:1n-9, 18:2n-6. Ascorbic acid content increased 10-fold from hatch to the end of Stage I (36 and 333 ug g-1 dw, respectively), while the content at the end of Stage II was higher in fed than starved larvae (439 and 174 ug g-1 dw, respectively). Our study will assist in the development of alternatives to nutritionally incomplete diets, such as live ongrown Artemia, to meet the requirements of phyllosoma in culture.

Item Type: Article
Authors/Creators:Ritar, AJ and Dunstan, GA and Crear, BJ and Brown, MR
Keywords: Artemia; Ascorbic acid; Fatty acid; Jasus edwardsii; Nutrition; Phyllosoma; Spiny lobster; Starvation
Journal or Publication Title: Comparative Biochemistry and Physiology Part A
DOI / ID Number: 10.1016/S1095-6433(03)00167-3
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