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Kinetic performance potential of different column formats and separation modes for liquid chromatography


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Causon, TJ (2012) Kinetic performance potential of different column formats and separation modes for liquid chromatography. PhD thesis, University of Tasmania.

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This thesis deals with theoretical and experimental studies on optimisation of separation
systems performed using liquid chromatography. The two major themes of work were
improving the quality of separations by maximising efficiency with respect to the analysis
time (kinetic performance) and by using temperature as an experimental variable. These
themes were explored for a range of separation modes and column formats utilised in
liquid chromatography.
Included in this thesis is a review of the principles and practices of kinetic performance
optimisation for liquid chromatography. Detailed concepts covered in this thesis are
introduced and future directions for chromatographic supports discussed.
The role of elevated temperatures (up to 160°C) for practical and performance benefits for
organic polymer monolithic stationary phases is examined for typical biomolecule
applications. Temperature variation, particularly rapid temperature pulsing, during the
separation process is examined using ion-exchange and hydrophilic interaction liquid
chromatography modes with capillary columns and a resistively heated column module.
The current performance limitations for the analysis of small anions by ion-exchange
chromatography are explored. Additional in silico data are used to transform experimental
isocratic data to gradient performance predictions for any normalised ramp rate and
physicochemical conditions. Examples of high efficiency (25,000-40,000 theoretical
plates) and sample peak capacity (nc = 84) were realised and closely matched predicted
A theoretical study of the open tubular column format for liquid chromatography with a
thick, porous layer of stationary phase shows the potential of this column format to improve chromatographic performance with a trade-off for mass loadability. It can be
shown that total column diameters should be less than 6 μm with column lengths typically
greater than 0.8 m for N values in the range of 125,000–500,000 at a maximum pressure of
400 bar. Elevated temperature LC (90°C) is also shown to increase the allowable total
column diameter to up to 9 μm for a larger range of N values (100,000–880,000).

Item Type: Thesis (PhD)
Keywords: liquid chromatography, performance, monolythic colums, open-tubulat LC, high temperature LC
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Copyright 2012 the author

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The main texts are published articles that cannot be made available from this repository. This record contains links to the articles at the publishers' websites.

Date Deposited: 13 Jan 2013 23:45
Last Modified: 28 Jul 2017 00:28
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