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Proteomics analysis for Barrett's Oesophagus biopsies and oesophageal lesions in HPV infected individuals

Obaid, N 2013 , 'Proteomics analysis for Barrett's Oesophagus biopsies and oesophageal lesions in HPV infected individuals', Coursework Master thesis, University of Tasmania.

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Oesophageal lesions caused by HPV are known recently as a contributor to oesophageal squamous cell carcinoma. Some evidences show that these lesions in association with Barrett’s oesophagus can progress to oesophageal adenocarcinoma. In addition, these lesions are considered to be precancerous or thought to have a role in progression to cancer by a synergistic effect.
Aims: To identify proteins that are related to pathological lesions of the oesophagus (Barrett's oesophagus and oesophageal HPV lesion) by using proteomic analysis. Methods: Four oesophageal biopsies were taken from patients with Barrett's oesophagus, HPV lesions and oesophagitis. Two biopsies were previously confirmed to have HPV lesions using a PCR technique. Proteomics analysis was undertaken by LC/MS/MS using the MudPIT technique.
Results: Two comparison groups of the samples were generated to perform a quantitative analysis for
this proteomics approach. The first comparison was between Barrett's oesophagus with HPV and
Barrett's oesophagus without HPV. Eleven proteins in this group showed significant association with
diseases, including cancers. In addition, one protein was identified previously as a Barrett's
oesophagus marker. The second comparison was between an oesophageal HPV lesion and an
oesophagitis sample. Five proteins showed potential significance in relation to cancers. Conclusion:
HPV lesions have over expression of proteins that are related to neoplasia when HPV is associated
with Barrett's oesophagus. This generates a hypothesis: HPV infection of the oesophagus could not
affect protein expression unless associated with Barrett's oesophagus. Many questions have arisen
and need further investigation.

Item Type: Thesis - Coursework Master
Authors/Creators:Obaid, N
Keywords: HPV, Barette's Oesophagus, Proteomics, pre-cancerous.
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