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Conjugation of benzoic acid in marsupials

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Awaluddin, Almah binti (1983) Conjugation of benzoic acid in marsupials. Coursework Master thesis, University of Tasmania.

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Abstract

The fate of benzoic acid was studied in 7 species of marsupials
and the echidna. Radiolabelled [7-14C]-benzoic acid was
administered to 3-7 animals of each marsupial species (brown
bandicoot, barred bandicoot, Tasmanian devil, potoroo,
pademelon, brush-tailed possum and sugar glider) and urine
was collected over ice for 24 hours.
Metabolites were extracted from acidified urine with ethyl
acetate and separated on fluorescent silica gel TLC plates.
Bands were visualised under UV, and eluted with methanol.
After methylation with diazomethane, benzoic acid, hippuric
acid and β-hydroxyphenylpropionic acid were identified by
GC/MS. The methylated benzoyl glucuronide required
trimethylsilylation before GC/MS. Quantification was by liquid
scintillation spectrometry. It was found that all species excreted most of the dose in
24 hr. As with eutherians, the major metabolite was hippuric
acid, with smaller amounts of benzoic acid and β-hydroxyphenylpropionic
acid. Benzoyl glucuronide excretion varied
greatly, from 2% of the dose in the pademelon to 32% in sugar
gliders. Similar variability has also been reported in
eutherians.

Item Type: Thesis (Coursework Master)
Keywords: Benzoic acid, Marsupials
Copyright Holders: The Author
Copyright Information:

Copyright 1983 the Author - The University is continuing to endeavour to trace the copyright
owner(s) and in the meantime this item has been reproduced here in good faith. We
would be pleased to hear from the copyright owner(s).

Additional Information:

Thesis (M. Pharm) - University of Tasmania, 1983. Bibliography: leaves 83-106

Date Deposited: 25 Nov 2014 00:43
Last Modified: 07 Jun 2016 04:51
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