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Analysis of the breast cancer proteome

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Bhatia, K (2006) Analysis of the breast cancer proteome. Research Master thesis, University of Tasmania.

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Abstract

PURPOSE
The discovery of the oestrogen and her-2 receptor proteins paved the way for the
subsequent development of adjunctive therapeutic options for the treatment of breast
cancer. The advancement of proteomic technology has enabled the identification of
such unique proteins differentially expressed in a range of disease states including
breast cancer. It is our aim to use this technique to identify potential target molecules
for future therapy.
METHODS
Breast cancer samples weighing 100mg were obtained from patients in Tasmania and
Greece. Normal breast tissue was obtained as control from patients undergoing
reduction mammoplasty. Sample preparation was performed using a sequential
extraction protocol to yield two solutions per tissue sample. This was followed by
two-dimensional gel electrophoresis and resultant gels analyzed using PDQuest
software.
RESULTS
Quantitative analysis revealed a 5-fold increased expression of 54 spots and decreased
expression of 20 spots in breast cancer versus normal tissue using solution 2 and 3
extracted gel images. All such identified spots were located within pI (Iso-electric
point) 6.0-8.2 and molecular weight 25-75kDa. Further detailed analysis enabled the
identification of a number of spots showing differential expression by clinical stage
including metastatic cancer, histological subtype, racial subgroup and amongst
familial cancers.
CONCLUSION
The further characterization of identified spots will involve the exclusion of known
markers using immunoblotting techniques with unknown spots subjected to mass
spectrometric analysis or amino acid sequence determination. This approach may
allow the identification of new diagnostic and/or therapeutic targets.

Item Type: Thesis (Research Master)
Keywords: Breast, Cancer in women, Biochemical markers
Copyright Holders: The Author
Copyright Information:

Copyright 2006 the Author - The University is continuing to endeavour to trace the copyright
owner(s) and in the meantime this item has been reproduced here in good faith. We
would be pleased to hear from the copyright owner(s).

Additional Information:

Available for library use only and copying in accordance with the Copyright Act 1968, as amended. Thesis (MMedSc)--University of Tasmania, 2007. Includes bibliographical references

Date Deposited: 25 Nov 2014 00:56
Last Modified: 16 Feb 2017 00:30
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