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Abstract

The analysis of milk lipids, especially ceramide, by thin layer
chromatography is described. The variation of ceramide in milk and
milk products under different external conditions is noted. The
fatty acid composition of milk ceramide and sphingomyelin is determined
by gas-liquid chromatography of the trimethylsilyl derivatives after
separation by TLC followed by hydrolysis. Quantitative methods of
analysis of ceramides are developed, and the ceramide in milk and milk
products estimated by picrylsulphonic acid. Finally, some synthetic
ceramides are prepared and analysed by TLC, infra red spectroscopy
and mass spectroscopy, the methods to be used (with GLC) in the future
for the complete analysis of the varied components of milk ceramides.
Some anomalous TLC spots are observed with structures possibly
different from the usual straight-chain form of ceramide.

Item Type:	Thesis - Coursework Master
Authors/Creators:	George, Edwin Ernest
Keywords:	Milk, Lipids
Copyright Holders:	The Author
Copyright Information:	Copyright 1975 the Author - The University is continuing to endeavour to trace the copyright owner(s) and in the meantime this item has been reproduced here in good faith. We would be pleased to hear from the copyright owner(s).
Additional Information:	Bibliography: l. 82-84. Thesis (M.Sc.) - University of Tasmania, 1975
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