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Molecular markers for abalone research


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Evans, Bradley Scott 2001 , 'Molecular markers for abalone research', PhD thesis, University of Tasmania.

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The Haliotidae is a family of marine gastropod molluscs, of the Order Archaeogastropoda
(Schremp 1981). The world-wide family consists of 56 currently described species from
temperate and tropical waters of both hemispheres. Approximately 25 abalone species are
currently harvested commercially from at least 15 countries, with many more taken by
recreational fisherman.
This thesis examines the utility of molecular genetic markers in abalone research, with particular
emphasis to the Australian fishery and aquaculture industries. The development of microsatellite
markers in temperate abalone, and their use in stock structure studies of abalone fisheries in
Australia and South Africa are presented.
Twenty-two microsatellite loci were isolated from a Haliotis rubra partial genomic library, and
their conservation in 12 other Haliotis species is presented. A maximum of 15 of these markers
were retained in the most closely related species, Haliotis conicopora, but the species status of
this group is questioned. Only 3 of the microsatellite loci examined were retained in each of two
North American species, Haliotis corrugata and H. fulgens. Methods for the detection of
microsatellite loci in other species is also discussed.
A study of genetic variability in H. rubra at eight microsatellite loci in seven Tasmanian, one
Victorian and two New South Wales locations is presented. These locations represent both fine (5
km) and broad (> 500 km) scale separation, and reveal a mean He of 0.552, with significant
genetic differentiation between Tasmanian samples and those from mainland Australia (F cT =
0.003; P < 0.0001). No significant differentiation was identified between Tasmanian samples, or
between mainland samples. Significant departures from Hardy-Weinberg equilibrium were
common, in all instances due to an excess of homozygotes. Evidence for the existence of null
alleles at two loci is presented as a factor in the departures from He.

Item Type: Thesis - PhD
Authors/Creators:Evans, Bradley Scott
Keywords: Abalone fisheries, Abalone culture, Abalone populations, Genetic markers, Microsatellites (Genetics)
Copyright Holders: The Author
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Copyright 2001 the Author - The University is continuing to endeavour to trace the copyright owner(s) and in the meantime this item has been reproduced here in good faith. We would be pleased to hear from the copyright owner(s).

Additional Information:

Thesis (Ph.D.)--University of Tasmania, 2002. Includes bibliographical references

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