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The chemistry and analysis of sphingolipids in natural products

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George, Edwin Ernest (1976) The chemistry and analysis of sphingolipids in natural products. Unspecified thesis, University of Tasmania.

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Abstract

Methods used for the analysis of both synthetic and natural
sphingolipids and their components are described.
Analytical data is given on ceramides derived from bovine-brain
cerebrosides, and on the sphingolipids derived from milk from a
predominantly Fresian herd. The major fatty acids in brain
sphingolipids are 24:0, h24:0, 22:0, h22:0, 18:0 and h18:0; 24:1 is
a less major component in brain cerebrosides, but a major component
in brain sphingomyelin. The milk sphingolipids comprise mainly
sphingomyelin, ceramide glucoside, and ceramide lactoside. All
three sphingolipids contain both non-hydroxy and hydroxy fatty acids,
and their compositions are similar; 23:0, 24:0, 22:0 and 16:0 (and
the corresponding hydroxy fatty acids) predominate, with 16:0
(or h16:0) and 24:1 (or h24:1) present to a lesser degree.
The separation of diastereoisomeric ceramides by TLC on borate-impregnated
silica gel, and by GLC, is described. Synthetic
ceramides are prepared for this work by direct coupling of long chain
base and fatty acid in the presence of a carbodiimide. Side-reaction
products produced by this method are analysed, accounting for
previously unknown TLC spots present in the crude preparations.
Finally the analysis of human serum lipids by spectrodensitometry
using phosphomolybdic acid is described. The methods are applied to
serums obtained from 68 apparently healthy adults, and quantitative
results given for ceramides, monoglycerides, and cholesterol (free
and total).

Item Type: Thesis (Unspecified)
Keywords: Sphingolipids
Copyright Holders: The Author
Copyright Information:

Copyright 1976 the Author - The University is continuing to endeavour to trace the copyright
owner(s) and in the meantime this item has been reproduced here in good faith. We
would be pleased to hear from the copyright owner(s).

Additional Information:

Being a progress report on research carried out up to 31.12.1975. Interim report as candidate for Ph.D.--University of Tasmania, 1976. Includes bibliographical references

Date Deposited: 09 Dec 2014 00:08
Last Modified: 22 Aug 2016 01:03
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