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Aspects of the Striped Trumpeter (Latris lineata) Immune Response

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Covello, Jennifer Marie (2010) Aspects of the Striped Trumpeter (Latris lineata) Immune Response. Unspecified thesis, University of Tasmania.

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Abstract

The striped trumpeter (Latris lineate Forester 1801) has been identified as a
new species for commercial culture in Tasmanian waters. Over the past 20 years,
much research has gone into understanding the biology of this species, with
specific attention to closing the life cycle and attaining good growth and survival
during the early developmental stages. These endeavours have been successful,
with the first cohort of juveniles recently transferred to sea cages for grow-out.
However, if the culture of striped trumpeter is to intensify, it will be important to have
an understanding of how its immune system functions, so that when disease issues
arise, they can be dealt with in a prompt and effective manner. To that end, the
broad aims of this thesis were (1) to investigate the ontogeny of adaptive immune
response at both the protein and mRNA level, (2) to investigate the inflammatory
response in terms of the mRNA expression levels of three important proinflammatory
cytokines and (3) to link the knowledge gained from the first two aims,
and investigate the influence of a recombinant interleukin-6 (rIL-6) on the proinflammatory
cytokine and immunoglobulin mRNA expression levels. The antibody
molecule (IgM) was characterised with regard to its structure and ontogeny. It was
discovered that the striped trumpeter possess IgM as a secreted tetramer, like that
of other teleost species. IgM protein did not reach detectable levels until 225 dph
using Western blot analysis, but transcripts of IgM mRNA were present at 5 dph via
RT-PCR. As the appearance of mRNA transcripts was earlier than in other marine
teleosts studied, the idea of maternally transferred IgM mRNA transcripts was
investigated. Additionally, three important pro-inflammatory cytokines, TNF-a, IL-
113, and IL-8, were cloned and sequenced for the first time in this species. Their
mRNA expression levels were compared between healthy and ectoparastized fish
using quantitative real-time PCR. It was found that striped trumpeter are able to
mount a strong inflammatory response at the site of parasite attachment and that a
somewhat less defined response occurrs systemically. Finally, the pleiotropic
cytokine, IL-6, was cloned and sequenced, and a recombinant protein generated.
The recombinant protein was used to investigate the influence of IL-6 on both the
inflammatory response and the Th2 pathway.

Item Type: Thesis (Unspecified)
Copyright Holders: The Author
Additional Information:

Includes bibliography

Date Deposited: 09 Dec 2014 00:16
Last Modified: 11 Mar 2016 05:53
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