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Studies on some lipids of clinical interest

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Mejglo, Zenon Antoni (1977) Studies on some lipids of clinical interest. PhD thesis, University of Tasmania.

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Abstract

Chapter I reports studies on a number of common drugs and
metabolites, some of which were found to affect lipids. Quantitative
studies of drug—induced release of cholesterol esters from lipoproteins
in vitro are described.
Tetracycline was found to act an both lipoproteins and proteins,
causing marked denaturation of the latter and a strong delipidation of
the former. Similar effects were noted in the cases of Atromid S and
Phenformin. These drugs were also reacted with serum in combination with
magnesium lactate alone, and in combination with pyridoxine, and in the
latter case some enhancement of delipidating effects were seen.
Similar tests on a number of amino acids were negative.
Spectrophotometric, electrophoretic and immunoelectrophoretic
investigations of the type and degree of binding of tetracycline and
nitrofurantoin to serum proteins are reported in Chapter II. Competitive
binding of tetracycline with displacement of lipids from their binding
sites support the findings of Baer, Palmer Schmitt and Chargaff.
Evidence is presented on competition between serum protein fractions for
the denaturing drug.
The main aim of the work described in Chapter III was to
ascertain whether chemical features of serum sphingolipids are of diagnostic
value. Quantitation of free ceramides showed that, while total serum
ceramides (which correlate well with serum triglycerides) seldom vary
beyond normal limits, fractions rich in hydroxy-acids indicate atherosclerotic
risk. Such diagnostic data appear more reliable than indications
from cholesterol assays.
The free serum ceramides noted were assigned origins from
sphingomyelin and glycosphingolipids respectively. A routine method for
the assay of free serum ceramides has been developed; it is based on a
thin—layer chromatographic technique followed by densitometric scanning.
The patterns of fatty acid moiety in diagnostically interesting
free serum ceramides have been established by gas—liquid chromatography.
The concomitant variation of base moieties appears of less practical
interest.

Item Type: Thesis (PhD)
Keywords: Lipids, Lipids, Cholesterol
Copyright Holders: The Author
Copyright Information:

Copyright 1977 the Author - The University is continuing to endeavour to trace the copyright
owner(s) and in the meantime this item has been reproduced here in good faith. We
would be pleased to hear from the copyright owner(s).

Additional Information:

Thesis (Ph.D.)--University of Tasmania, 1977. Includes bibliographical references

Date Deposited: 19 Dec 2014 02:23
Last Modified: 02 Oct 2017 06:46
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