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Development of a technique for fish feed digestibility estimations using microtracers


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Kabir, N M Jahangir 1993 , 'Development of a technique for fish feed digestibility estimations using microtracers', Research Master thesis, University of Tasmania.

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The aim of this thesis was to compare the methods of feed digestibility
determination in fish and subsequently develop a technique for fish feed
digestibility estimations using microtracer. The review of current methods
showed that none of the existing methods fulfil adequately the criterion of
an ideal method when employing external markers.
Therefore microtracer F-Ni was used in this study and a series of
experiments was conducted to evaluate the digestibility coefficients in fish
( rainbow trout, Oncorhynchus mykiss) using microtracer F-Ni as a marker
and compare its suitability with the conventional Cr203 and acid insoluble
ash (AIA) markers.
The particle counts of microtracer F-Ni per unit weight were validated in
Experiment 1, as it was essential to establish a consistent count of particles
per unit weight for the calculation of digestibility values. Both direct
counting and colour development methods were used for this purpose.
Results showed that microtracer F-Ni marker contained on average 64
particle per mg. The sequential uniformity in the rate of passage of the microtracer through
the digestive tract were tested in Experiment 2, using similar diets with
four inclusion levels to select one level for further digestibility studies. One
percent inclusion level was found to be the best as the rate of increase in
concentration in different parts of the digestive tract and feces were
sequential and progressive for all the three days tested.
Apparent digestibility coefficients (ADC) of protein and dry matter were
determined and compared employing different fecal collection procedure
and different collection interval using this marker with chromic oxide marker and acid insoluble ash marker in Experiment 3. Results indicated
that the ADC values generated by using microtracer F-Ni were consistent
and in close agreement with the ADC values calculated by using chromic
oxide as marker in all the situations. The ADC determined by using AIA
were inconsistent and higher than the ADC calculated by both chromic
oxide and microtracer F-Ni markers. When tnicrotracer F-Ni and chromic
oxide were incorporated into the same diet, ADC values calculated were
higher than values obtained with individual marker inclusion.
In Experiment 4, protein and dry matter digestibility of fish meal and
sunflower meal ingredients were determined and compared using
microtracer F-Ni, Cr203 and AIA markers. Digestibility coefficients of
nutrients generated using microtracer resulted ADC values similar (P>
0.05) to those using chromic oxide as external marker. Experimental diets
containing either microtracer F-Ni or chromic oxide supported excellent
growth performances and feed conversion efficiency.
In summary, microtracer F-Ni incorporated at 1% can be used as an
external marker in fish feed digestibility studies in rainbow trout.

Item Type: Thesis - Research Master
Authors/Creators:Kabir, N M Jahangir
Keywords: Fishes, Fishes, Fishes
Copyright Holders: The Author
Copyright Information:

Copyright 1993 the Author - The University is continuing to endeavour to trace the copyright
owner(s) and in the meantime this item has been reproduced here in good faith. We
would be pleased to hear from the copyright owner(s).

Additional Information:

Includes bibliographical references (leaves 142-153). Thesis (M.App.Sc.)--University of Tasmania, 1994

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