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The effect of temperature on protein synthesis and growth efficiency in juvenile barramundi, Lates calcarifer

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Katersky, Robin Sue (2007) The effect of temperature on protein synthesis and growth efficiency in juvenile barramundi, Lates calcarifer. PhD thesis, University of Tasmania.

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Abstract

Temperature has a marked and direct effect on key physiological processes in fish, each species has a range of temperatures over which it survives (thermal tolerance range) and where growth occurs. Culture of barramundi, Lates calcarifer, is occurring at temperatures across the thermal tolerance (15-40°C). Consequently, this thesis examined the effect of temperature on protein metabolism: feed intake (FI, g.d⁻¹), growth (SGR, %.d⁻¹), growth efficiency (in relation to protein and energy retention) and protein synthesis (ks, %.d⁻¹) were measured. Fish were fed to satiation daily on the same diet. Protein synthesis was determined using the flooding dose method with ³H-phenylalanine.
Models of Fl, SGR and growth efficiency were developed from two experiments.
The first examined temperatures from 21-33°C at 3°C intervals and the second looked at temperatures 27, 33, 36 and 39 °C. These models calculate the optimal
temperature for each parameter to be 31°C. However, the optimal range for maximum growth efficiency (both PPV and PEV) is estimated to be 10 times wider than for Fl or SGR. These models also predict an optimal temperature which is —4°C higher than what has been previously determined for barramundi. The broad optimal range for growth efficiency is much more extensive than previously recognized for barramundi.
Experiments were conducted to quantify the effect of temperature on protein synthesis (ks). The first experiment examined white muscle (WM) and whole body (WB) lc, from 21-33°C, at 3°C intervals, 24 h after feeding. No significant differences existed between temperatures, however regardless of temperature WBk, was —4 times greater than WMIcs. A second experiment was conducted at 21, 27 and 33°C on the WM, Liver and WBk, at times 0, 4, 8, 12 and 24 h after feeding. At the 27 and 33°C WMks peaked 6 h after feeding and remained significantly elevated over the initial 12 h. A final experiment was conducted at 27, 33, 36 and 39°C on the WM, Liver, stomach, digestive tract and WBks at times 0, 2, 4, 6, 8, 12, and 24 h
after feeding. No differences occur between WBk, at 27 and 33°C and peaks occur —3 h after feeding; liver ks peaks 2 h after feeding and is an order of magnitude higher than WBks. At 21 and 39°C, WBIcs is not affected by feeding and this corresponds to the low growth rates observed at these temperatures. The results of the three protein synthesis experiments have been combined with protein growth data to model protein turnover over the thermal tolerance range for juvenile barramundi.

Item Type: Thesis (PhD)
Keywords: Giant perch, Giant perch
Copyright Holders: The Author
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Copyright 2007 the Author - The University is continuing to endeavour to trace the copyright
owner(s) and in the meantime this item has been reproduced here in good faith. We
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Available for library use only and copying in accordance with the Copyright Act 1968, as amended. Thesis (PhD)--University of Tasmania, 2007. Includes bibliographical references

Date Deposited: 19 Dec 2014 02:50
Last Modified: 09 May 2017 05:08
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