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Initial defence responses in sapwood of Eucalyptus nitens (Maiden) following wounding and fungal inoculation


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Barry, KM, Pearce, RB, Evans, SD, Hall, LD and Mohammed, CL 2001 , 'Initial defence responses in sapwood of Eucalyptus nitens (Maiden) following wounding and fungal inoculation' , Physiological and Molecular Plant Pathology, vol. 58, no. 2 , pp. 63-72 , doi: 10.1006/pmpp.2000.0314.

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Xylem defence responses occurring in pot-grown Eucalyptus nitens(Maiden) saplings were analysed within the first few weeks following stem wounding and inoculation with the non-aggressive decay fungus Ganoderma adspersum(Schulz). The fungus colonized the dead xylem cells up to 5mm above and below the wound after 30 days. Evidence of xylem cell death and discolouration was detectable within 12h and vessel tyloses were present after 3 days. Concentration of tetra-galloylglucose increased by 3.5-fold within 24h, while the trend for total phenols was a slow increase reaching a maximum after 21 days. Suberin was not detected. Early accumulation of water content has been proposed as a defence component of host-pathogen interactions in woody angiosperm xylem, but this was not obvious for E. nitens. Nuclear magnetic resonance imaging (NMR imaging or MRI) was used to measure small-scale distributions in moisture content around the inoculated stem wounds in intact E. nitens saplings. Some moisture accumulation was detectable at lesion margins of wounds analysed after 3 weeks. However, this was not detectable by gravimetric measurements. This increase in moisture content may be a consequence of wound physiology rather than an active defence response.

Item Type: Article
Authors/Creators:Barry, KM and Pearce, RB and Evans, SD and Hall, LD and Mohammed, CL
Keywords: Injury ; Lesion ; Initial condition ; Inoculation ; Mycosis ; Defensive response ; Colonization ; Histopathology ; Infection modality ; Chemical modification ; Pathophysiology ; Water content ; Zone ; Sapwood ; Stem ; Xylem ; Antimicrobial agent ; Phenols ; Experimental study ; Moisture measurement ; Non invasive method ; Nuclear magnetic resonance imaging ; Eucalyptus nitens ; Ganoderma adspersum ; WOOD DECAY ; Infection ; Forestry ; Measurement method ; Physical method ; Mycology ; Host agent relation ; Basidiomycetes ; Fungi ; Thallophyta ; Myrtaceae ; Dicotyledones ; Angiospermae ; Spermatophyta ; Hardwood forest tree ; Wood destroying fungi ; Woody plant ; FOREST PATHOLOGY ; Eucalyptus nitens; tree; Ganoderma adspersum; antim
Journal or Publication Title: Physiological and Molecular Plant Pathology
DOI / ID Number: 10.1006/pmpp.2000.0314
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