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Proteomic analysis of breast cancer

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Roberts, Kylie Maree 2009 , 'Proteomic analysis of breast cancer', PhD thesis, University of Tasmania.

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Abstract

Introduction: Breast cancer is a disease that will affect every woman in her life.
Whether that affect will be direct or indirect is, at this stage almost impossible to
determine. There have been several key findings in recent years with genes being found
that are linked directly to breast cancer. With these being attributed to less than 10% of
breast cancers diagnosed, the need for new biomarkers of breast cancer exists. This
study aims to find new biomarkers though differential analysis of breast cancer tissue.
Several different analysis have been conducted to provide insight in to potential new
markers for diagnosing early onset breast cancers, looking for signs in otherwise healthy
tissue and ascertaining differences between cancer and healthy tissue as a whole. This
study also looks in to the functional areas of proteomic research, determining a need for
further investigation in to IGF-1 and ~-casein, which have been inconclusively linked to
breast cancer in the past.

Methods: By employing selected proteomic technologies including iso-electric
focusing and polyacrylamide gel electrophoresis and combining samples from
populations from different locations across the globe, collections of two-dimensional
gels from breast tissue were differentially compared to isolate proteins that have a high
likelihood of being involved in breast cancer.

Results: This approach to breast cancer research has led to many proteins being isolated
that have a role in breast cancer. The proteins found have a diverse range of roles from
signaling proteins to structural proteins. Within those proteins successfully identified
are proteins like HSP60, known to have a role in breast cancer, serum albumin which
has limited information regarding a role in breast cancer and FLJ20309, one of several
proteins that have been assumed from cDNA clones with little available information
available regarding its function.

Conclusions: This study has led to the discovery of proteins that were previously
thought to have limited, if no input in breast cancer. All of the proteins found require
further experiments to fully elucidate their function in breast cancer however at least
some of these are show promise as being of diagnostic and/or therapeutic value.

Item Type: Thesis - PhD
Authors/Creators:Roberts, Kylie Maree
Keywords: Breast, Cancer in women, Biochemical markers
Copyright Holders: The Author
Copyright Information:

Copyright 2009 the author - The University is continuing to endeavour to trace the copyright
owner(s) and in the meantime this item has been reproduced here in good faith. We
would be pleased to hear from the copyright owner(s).

Additional Information:

Available for use in the Library and copying in accordance with the Copyright Act 1968, as amended. Thesis (PhD)--University of Tasmania, 2009. Includes bibliographical references

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