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Langerhans dendritic cells, tobacco smoke and lung tumours


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Zeid, Naiem A 1995 , 'Langerhans dendritic cells, tobacco smoke and lung tumours', PhD thesis, University of Tasmania.

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The distribution of Langerhans dendritic cells in tobacco smoke related human
lung cancer, tobacco smoke induced Langerhans cells changes during cutaneous
and pulmonary carcinogenesis in mice were investigated. While dendritic cells
were rare in normal lung, their density was marked in bronchioalveolar, well and
moderately differentiated squamous cell carcinomas. Small cell lung cancer and
poorly differentiated squamous cell carcinoma showed the lowest density. High
density of dendritic cells in lung tumours was associated with marked tumour
infiltrating lymphocytes and favourable survival.
Exposure of mice to tobacco smoke increased the density of zinc-iodide-osmium
positive pulmonary Langerhans cells. Pulmonary Langerhans cell granulomatosis
and a proliferative alveolar and bronchial epithelial tumour-like lesions were
observed. The density of pulmonary Langerhans cells returned to that of the
control level after ceasing exposure to tobacco smoke and the interstitial
granulomatous lesions disappeared, but the bronchial epithelial metaplasia did not
reverse. The ultrastructure of the zinc-iodide-osmium positive cells was similar to
that of pulmonary Langerhans cells.
Tobacco smoke condensate increased the density, changed the morphology and
impaired the function of epidermal Langerhans cells. This was associated with skin
tumour development.
While examining human lung lesions S100 positive DC were observed migrating
into tumours. So these cells and their relation to LDC were further examined in
blood. Metrizamide enriched dendritic cells isolated from human blood, were S100
positive, co-expressed CD45 and were non-phagocytic. Their diameter was larger
than that of CD3 positive T lymphocytes and smaller than CD14 positive
monocytes. The ultrastructural features were consistent with that of blood
dendritic cells. Their number slightly decreased with age, equal numbers were
observed in both sexes and formed 0.09 ± 0.07 cells x 10 9 per litre of blood.

Item Type: Thesis - PhD
Authors/Creators:Zeid, Naiem A
Keywords: Langerhans cells, Tobacco smoke, Lungs, Carcinogenesis
Copyright Holders: The Author
Copyright Information:

Copyright 1995 the Author - The University is continuing to endeavour to trace the copyright owner(s) and in the meantime this item has been reproduced here in good faith. We would be pleased to hear from the copyright owner(s).

Additional Information:

Includes bibliographical references (leaves 130-175). Thesis (Ph.D.)--University of Tasmania, 1996

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