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Amoebic gill disease of Atlantic salmon : resistance, serum antibody response and factors that may influence disease severity

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Vincent, BN (2008) Amoebic gill disease of Atlantic salmon : resistance, serum antibody response and factors that may influence disease severity. PhD thesis, University of Tasmania.

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Abstract

Amoebic gill disease (AGD) is a condition of some marine-cultured fish worldwide and
is the result of Neoparamoeba spp. infection. If AGD-affected fish are left untreated,
major mortalities can occur. In Tasmania, Australia, fresh water bathing remains the only
treatment for AGD, a practice that represents approximately 10-20% of production costs.
Therefore, development of a preventative measure such as vaccination is a priority for
Tasmanian salmon growers. In this project, resistance of Atlantic salmon to AGD and the
development of a serum antibody response to Neoparamoeba spp. were assessed. Sera
from AGD-affected Atlantic salmon were screened to identify potential candidate
antigens for an AGD vaccine. Atlantic salmon exposed to Neoparamoeba spp. and
subsequently challenged with AGD demonstrated resistance in terms of increased
survival compared to AGD-narve fish. In addition, antibodies that bound cell-surface
carbohydrate epitope(s) of Neoparamoeba spp. were detected in the sera of some fish
after secondary exposure to Neoparamoeba spp .. In light of this, further screening of sera
from Atlantic salmon exposed to Neoparamoeba spp. in the laboratory or during sea-cage
culture was conducted. Antibodies present in the sera of some AGD-affected Atlantic
salmon predominately bound carbohydrate residues expressed on the cell-surface of
Neoparamoeba spp.
Ideally an AGD vaccine would contain peptide antigen(s) that can easily be produced by
recombinant DNA technology. Therefore, an alternative approach to identify candidate
vaccine antigens for an AGD vaccine was needed. Some pathogenic amoebae colonise host tissues via lectin-mediated attachment and lectins have shown promise as candidate
vaccine antigens. The in vivo effect of mucus and saccharides on the ability of
Neoparamoeba spp. to cause AGD was investigated. The number of AGD lesions was
significantly reduced when amoebae were incubated in mucus or any of the range of
saccharides assessed. These data suggest that colonisation of Neoparamoeba spp. on gill
tissues of Atlantic salmon may be lectin-mediated and the ensuing infection can enhance
resistance to Neoparamoeba spp. However, very few AGD-affected Atlantic salmon
develop a serum antibody response to Neoparamoeba spp. suggesting that the
development of antibody-mediated protection of Atlantic salmon during Neoparamoeba
spp. infection is unlikely.

Item Type: Thesis (PhD)
Keywords: Atlantic salmon, Fishes, Atlantic salmon fisheries, Gills, Salmon farming
Copyright Holders: The Author
Copyright Information:

Copyright 2008 the author

Additional Information:

Chapter 2 appears to be, in part, the equivalent of the peer reviewed version of the following article: Vincent, B. N., Morrison, R. N. and Nowak, B. F., (2006) Amoebic gill disease (AGD)affected Atlantic salmon, Salmo salar L., are resistant to subsequent AGD challenge, Journal of fish diseases 29, 549-559, which has been published in final form at http://dx.doi.org/10.1111/j.1365-2761.2006.00751.x This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving.

Chapter 3 appears to be, in part, the equivalent of the peer reviewed version of the following article: Vincent, B. N., Nowak, B. F. and Morrison, R. N., (2008) Detection of serum antiNeoparamoeba spp. antibodies in amoebic gill disease (AGD)-affected Atlantic salmon (Salmo salar L.). Journal of fish biology, 73, 429-435, which has been published in final form at http://dx.doi.org/10.1111/j.1095-8649.2008.01891.x This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving.

Chapter 4 appears to be the equivalent of the post-print version of the following article: Vincent, B. N., Adams, M. B., Nowak, B. and Morrison, R. N., (2008) Cell-surface carbohydrate antigen(s) of wild-type Neoparamoeba spp. are immunodominant in seacage cultured Atlantic salmon (Salmo salar L.) affected by amoebic gill disease (AGO), Aquaculture, 288(3-4), 153-158, which has been published in final form at http://dx.doi.org/10.1016/j.aquaculture.2008.11.036

Appendix 4 includes published versions of two articles which have been removed from the PDF. The remaining articles appear to be author submissions articles published in part from chapters 2 and 3.

Date Deposited: 04 Feb 2015 23:34
Last Modified: 23 Jun 2016 23:21
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