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Abstract

Capillary electrophoresis (CE) is regarded as a powerful separation technique that is an
alternative or complementary technique to more traditional methods such as gel
electrophoresis and liquid chromatography. When applied to the separation of inorganic
species, capillary electrophoresis still continues to take second place to other competitive
techniques such as ion chromatography (IC) and elemental mass spectrometry. CE is often
touted as having several obvious advantages over chromatographic techniques (mostly IC)
including high resolving power, speed, instrumental simplicity, flexibility and cost‐efficiency.
On the other hand, CE is frequently cited as having a number of comparative disadvantages
such as poor reproducibility and sensitivity. The work undertaken in this thesis describes
technical innovations to harness the inherent advantages of CE whilst minimising the
disadvantages as part of the development of a system for the rapid determination of common
small environmental anions and cations. It is unique in its capability to analyse directly from
a sample flow, making it especially attractive for monitoring purposes. To enable a move from
a capillary to a chip‐based system, simple, low cost techniques for the manufacture of
polymeric microchips and the incorporation of detection electrodes were developed using
limited resources to provide further improvements in speed and reduce resource
consumption. A multiplexed polymeric microchip system was developed employing a novel
hydrodynamic injection mechanism to reduce sample matrix effects and injection bias, and
to improve the quantitative performance of the system. Finally, a compact multipurpose
microfluidic platform is developed to support future research interests.

Item Type:	Thesis - PhD
Authors/Creators:	Gaudry, AJ
Keywords:	Capillary electrophoresis, Microfluidics, Simultaneous separation, Microchip.
Copyright Information:	Copyright 2014 the author
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