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Using SSR markers for hybrid identification and resource management in Vietnamese Acacia breeding programs

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Le, S Using SSR markers for hybrid identification and resource management in Vietnamese Acacia breeding programs. [Dataset] (Submitted)

[img] Microsoft Excel (Microsatellite genotypes of Acacia samples used in Son Le 2017 paper in TGG (Tree Genetics & Genomes)) - Other
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Abstract

We used a set of 16 SSR markers to check the identity of pure-species and hybrid clones in Vietnam’s Acacia auriculiformis, A. mangium and acacia hybrid (A. mangium x A. auriculiformis) breeding programs. The statistics package HIest, applied to a large synthesized population, enabled accurate allocation of genotypes to the two pure species, F1 and F2 interspecific hybrids and backcrosses, based on estimates of hybridity and heterozygosity. The hybridity status of putatively pure A. mangium and A. auriculiformis clones in adjacent clonal seed orchards was checked. Four out of 100 clones selected as A. mangium were found to be backcrosses (A. mangium x F1 interspecific hybrid) while out of 96 clones selected as A. auriculiformis two were F1 hybrids and two were backcrosses (A. auriculiformis x F1 hybrid). The markers were then applied to check the hybridity status of 160 putative acacia F1 hybrid genotypes that had been selected on morphological criteria from open-pollinated progenies collected from A. auriculiformis and A. mangium parents. Many selections based on morphology were found to be mistaken. Only thirteen of 63 clones originating from A. auriculiformis mothers were F1 hybrids, four were backcrosses and the remaining 46 were pure A. auriculiformis. Fewer mistakes were evident for clones selected from A. mangium mothers, with 82 out of 89 clones confirmed as F1 hybrids, three as backcrosses and four as pure A. mangium. The occurrence of F1 hybrids and backcrosses in pure species seed orchards and their progeny shows that inter-species contamination is an issue requiring management in both pure-species and hybrid breeding of these species in Vietnam. Examination of genetic distances among verified clones showed patterns of relatedness that were consistent with pedigree records. Implications for resource management as well as for breeding and clonal selection strategies are considered.

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DataCite citation

Le, S (2017): Using SSR markers for hybrid identification and resource management in Vietnamese Acacia breeding programs. University of Tasmania. Dataset. http://dx.doi.org/10.4226/77/594af15dba65a

Date Deposited: 21 Jun 2017 22:29
Last Modified: 21 Jun 2017 22:53
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