PDF TheLKA_gene.pdf | Request a copy Full text restricted Available under University of Tasmania Standard License.

Abstract

Brassinosteroids (BRs) are growth-promoting plant steroid hormones, and in garden pea (Pisum sativum L.), the lka mutant is defective in BR perception. Here, we show that LKA encodes P. sativum BRI1 (PsBRI1), a homolog of BRI1, which is the Arabidopsis leucine-rich repeat receptor-like kinase/BR receptor. PsBRI1 was isolated by screening a pea cDNA library using Arabidopsis BRI1 cDNA as the probe. PsBRI1 is predicted to encode a 1188-amino-acid protein that has 78% similarity with Arabidopsis BRI1. Sequence analysis of PsBRI1 in the lka mutant led to the identification of a missense mutation that converts the highly conserved aspartic acid residue to asparagine, which is
located in the leucine-rich repeat, just before the island domain that may bind BR or a BR-protein complex. The mutation identified in PsBRI1 co-segregated with the semi-erectoide lka phenotype. Transcript analysis of LKA/PsBRI1 indicates that it is ubiquitously expressed in pea and that the expression was downregulated by exogenous BR. The lka mutant was then utilized in further studies to analyze the independent actions of BR and gibberellin (GA) through the characterization of BR response on GA mutants and GA response on BR mutants.

Item Type:	Article
Authors/Creators:	Nomura, T and Bishop, GJ and Kaneta, T and Reid, JB and Chory, J and Yokota, T
Keywords:	brassinosteroid; receptor; insensitive mutant; pea; gibberellin
Journal or Publication Title:	Plant Journal
ISSN:	0960-7412
DOI / ID Number:	https://doi.org/10.1046/j.1365-313X.2003.01863.x
Additional Information:	The definitive version is available at www.blackwell-synergy.com
Item Statistics:	View statistics for this item

Actions (login required)

Item Control Page