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Multidimensional separation approaches for complex sample analysis : two-dimensional ion chromatography ✕ capillary electrophoresis

Ranjbar Shourabi, L ORCID: 0000-0002-9079-2296 2017 , 'Multidimensional separation approaches for complex sample analysis : two-dimensional ion chromatography ✕ capillary electrophoresis', PhD thesis, University of Tasmania.

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Abstract

The work presented in this thesis describes development, evaluation and
implementation of an online interfacing/modulation technique for comprehensive
two-dimensional ion chromatography ✕ capillary electrophoresis (IC ✕ CE) separation
of low-molecular-mass ionic or ionogenic compounds.
Chapter 1 provides an insight into the evolution of multidimensional liquid-phase
separations combining both chromatography and electrophoresis from inception in
1948 to present. As noted in this chapter, column-based multidimensional
electrophoretic and chromatographic separations have shown to be powerful
complementary techniques to the traditional two-dimensional gel-electrophoresis
approaches; especially for the analysis of lower molecular-mass-molecules in addition
to proteins and peptides. However, complexities in instrumentation and online
interfacing technologies are known to have hindered development of such techniques.
Chapter 2 introduces a non-focusing interfacing technology for online hyphenation
of ion chromatography with capillary electrophoresis. The system compromised of an
in-house sequential-injection capillary instrument which, through the use of a twoposition
six-port injection valve, allowed comprehensive sampling of the IC effluent
for quantitative analysis of inorganic anions and haloacetic acids in water.
In addition to instrumental developments, the second section of this thesis focuses on
alternative method development approaches for screening/optimisation of IC ✕ CE
separations.
Chapter 3 demonstrates the impact of background electrolyte pH on sample
dimensionality and peak capacity for IC ✕ CE separation of multi-valent lowmolecular-
mass organic acids. In this chapter, a simplified screening approach based
on calculation and maximising of Euclidean distance between the two-dimensional
peaks was used to predict the optimal background electrolyte pH in CE as well as the
eluent profile in IC. However, due to lack of overall two-dimensional selectivity,
further investigations were necessary to make full resolution of the analytes possible.
In Chapter 4, based on a ground set by the findings presented in Chapter 3, a
universal framework is proposed for simultaneous screening of separation selectivity
in both dimensions as a preceding step in method development/optimisation of twodimensional
separations. In this chapter, through the introduction of the concept of
two-dimensional selectivity mapping, effect of stationary phase in IC and background
electrolyte pH in CE on the overall two-dimensional separation selectivity was
studied to enable full resolution of the organic acids.
Finally, Chapter 5 of this thesis discusses the limitations of IC ✕ CE and provides
further insight and direction for future research.

Item Type: Thesis - PhD
Authors/Creators:Ranjbar Shourabi, L
Keywords: two-dimentional separations, ion chromatography, capillary electrophoresis, 2D separation selectivity
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Copyright 2017 the Author

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