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Direct production of microstructured surfaces for planar chromatography using 3D printing

Macdonald, NP ORCID: 0000-0001-6857-5458, Currivan, SA ORCID: 0000-0002-6984-2842, Tedone, L ORCID: 0000-0002-5252-8865 and Paull, B ORCID: 0000-0001-6373-6582 2017 , 'Direct production of microstructured surfaces for planar chromatography using 3D printing' , Analytical Chemistry, vol. 89 , pp. 2457-2463 , doi: 10.1021/acs.analchem.6b04546.

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Abstract

Through optimization of the printing process and orientation, a suitably developed surface area has been realized upon a 3D printed polymer substrate to facilitate chromatographic separations in a planar configuration. Using an Objet Eden 260VS 3D printer, polymer thin layer chromatography platforms were directly fabricated without any additional surface functionalization and successfully applied to the separation of various dye and protein mixtures. The print material was characterized using gas chromatography coupled to mass spectrometry and spectroscopic techniques such as infrared and Raman. Preliminary studies included the separation of colored dyes, whereby the separation performance could be visualized optically. Subsequent separations were achieved using fluorescent dyes and fluorescently tagged proteins. The separation of proteins was affected by differences in the isoelectric point (pI) and the ion exchange properties of the printed substrate. The simple chromatographic separations are the first achieved using an unmodified 3D printed stationary phase.

Item Type: Article
Authors/Creators:Macdonald, NP and Currivan, SA and Tedone, L and Paull, B
Keywords: 3D printing, separation science, additive manufacturing, planar chromatography
Journal or Publication Title: Analytical Chemistry
Publisher: Amer Chemical Soc
ISSN: 0003-2700
DOI / ID Number: 10.1021/acs.analchem.6b04546
Copyright Information:

Copyright 2017 American Chemical Society

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