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Assessing sequence diversity of groundnut rosette disease agents and the distribution of Groundnut rosette assistor virus in major groundnut-producing regions of Ghana

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Appiah, AS, Sossah, FL, Tegg, RS ORCID: 0000-0001-7668-7927, Offei, SK and Wilson, CR ORCID: 0000-0001-5434-3816 2017 , 'Assessing sequence diversity of groundnut rosette disease agents and the distribution of Groundnut rosette assistor virus in major groundnut-producing regions of Ghana' , Tropical plant pathology, vol. 42, no. 2 , pp. 109-120 , doi: 10.1007/s40858-017-0140-x.

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Abstract

The incidence of Groundnut rosette assistor virus (GRAV) in farmers’ fields and sequence diversity of groundnut rosette disease (GRD) agents were assessed in the three northern groundnut production regions of Ghana. GRAV incidence was high (69.5 to 75.0%) but not significantly different between the regions. Nucleotide sequencing of GRAV coat protein (CP) gene revealed 99–100% identity among the Ghanaian isolates and 97–100% similarity to GRAV sequences from Nigeria and Malawi for both nucleotide and predicted amino acids. Nucleotide sequence identities of partial ORF3 and 4 of Groundnut rosette virus (GRV) among the Ghanaian isolates were more variable (89–100%). Ghanaian GRV isolates were more closely related in nucleotide sequence identity to Nigerian isolates (95–98%) than Malawian isolates (88–90%). Similarly, nucleotide identity within Ghanaian GRV-sat RNA’s were close (94–100%), but distinct from Nigerian (82–87%) and Malawian (82–86%) GRV-sat RNAs. Ghanaian isolates of all three agents of GRD showed no obvious isolate diversity patterns based on the regions from where they were collected. We present the first report on the distribution and genetic diversity of GRD agents in Ghana.

Item Type: Article
Authors/Creators:Appiah, AS and Sossah, FL and Tegg, RS and Offei, SK and Wilson, CR
Keywords: Arachis hypogaea, groundnut rosette assistor virus, groundnut rosette virus, field survey, groundnut rosette disease, sequence analysis
Journal or Publication Title: Tropical plant pathology
Publisher: Springer
ISSN: 1982-5676
DOI / ID Number: 10.1007/s40858-017-0140-x
Copyright Information:

Copyright 2017 Sociedade Brasileira de Fitopatologia

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