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Vitamin D both facilitates and attenuates the cellular response to lipopolysaccharide

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Chen, L ORCID: 0000-0003-1897-9264, Eapen, MS ORCID: 0000-0003-0570-7059 and Zosky, GR ORCID: 0000-0001-9039-0302 2017 , 'Vitamin D both facilitates and attenuates the cellular response to lipopolysaccharide' , Scientific reports, vol. 7 , pp. 1-8 , doi: 10.1038/srep45172.

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Abstract

Vitamin D has a range of non-skeletal health effects and has been implicated in the response torespiratory infections. The aim of this study was to assess the effect of vitamin D on the response ofepithelial cells, neutrophils and macrophages to lipopolysaccharide (LPS) stimulation. BEAS-2B cells(airway epithelial cell line) and primary neutrophils and macrophages isolated from blood samples werecultured and exposed to LPS with and without vitamin D (1,25(OH)2D). The production of IL-6, IL-8,IL-1β and TNF-α of all cells and the phagocytic capacity of neutrophils and macrophages to E. coli wereassessed. Vitamin D had no effect on BEAS-2B cells but enhanced the production of IL-8 in neutrophils(p=0.007) and IL-1β in macrophages (p=0.007) in response to LPS. Both vitamin D (p=0.019) andLPS (p<0.001) reduced the phagocytic capacity of macrophages. These data suggest that the impactof vitamin D on responses to infection are complex and that the net effect will depend on the cellsthat respond, the key response that is necessary for resolution of infection (cytokine production orphagocytosis) and whether there is pre-existing inflammation.

Item Type: Article
Authors/Creators:Chen, L and Eapen, MS and Zosky, GR
Keywords: vitamin D, LPS, cytokines, neutrophils, macrophages, epithelial cells
Journal or Publication Title: Scientific reports
Publisher: Nature Publishing Group
ISSN: 2045-2322
DOI / ID Number: 10.1038/srep45172
Copyright Information:

Copyright 2017 The AuthorsLicensed under Creative Commons Attribution 4.0 International (CC BY 4.0)https://creativecommons.org/licenses/by/4.0/

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