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Abstract

Cytochrome P450s (CYP450s) are a rapidly evolving family of enzymes, making it difficult to identify bona fide orthologs with notable lineage-specific exceptions. In ecdysozoans, a small number of the most conserved orthologs include enzymes which metabolize ecdysteroids. Ecdysone pathway components were recently shown in a decapod crustacean but with a notable absence of shade, which is important for converting ecdysone to its active form, 20-hydroxyecdysone (20HE), suggesting that another CYP450 performs a similar function in crustaceans. A CYPome temporal expression analysis throughout metamorphosis performed in this research highlights several un-annotated CYP450s displaying differential expression and provides information into expression patterns of annotated CYP450s. Using the expression patterns in the Eastern spiny lobster Sagmariasus verreauxi, followed by 3D modelling and finally activity assays in vitro, we were able to conclude that a group of CYP450s, conserved across decapod crustaceans, function as the insect shade. To emphasize the fact that these genes share the function with shade but are phylogenetically distinct, we name this enzyme system Shed.

Item Type:	Article
Authors/Creators:	Ventura, T and Bose, U and Fitzgibbon, QP and Smith, GG and Shaw, PN and Cummins, SF and Elizur, A
Keywords:	CYP450, ecdysone, juvenile hormone, decapoda, molt, metamorphosis
Journal or Publication Title:	Journal of Steroid Biochemistry and Molecular Biology
Publisher:	Pergamon-Elsevier Science Ltd
ISSN:	0960-0760
DOI / ID Number:	10.1016/j.jsbmb.2017.04.007
Copyright Information:	Crown Copyright © 2017 Published by Elsevier
Related URLs:	UTAS Profile: Fitzgibbon, QP Google Scholar: Smith, GG UTAS Profile: Smith, GG
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