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Abstract

Hepatic gene expression and liver histology were examined in sand flathead (Platycephalus bassensis) from six locations in Port Phillip Bay, Victoria, Australia. Four sets of genes including thyroid-related genes (D1, D2, TTR, TRα and TRβ), metal metabolism-related genes (MT, MTF1, TF, Ferritin and FPN1), apoptosis-related genes (Diablo/SMAC1, Diablo/SMAC2 and CYP1A) and an endoplasmic reticulum stress biomarker gene (GRP78) were examined in female flathead using qRT-PCR. TRβ and Diablo/SMAC1 gene expression was significantly up-regulated in fish from all polluted sites compared to those from a reference site. The transcripts of TRα and FPN1 were significantly higher in flathead from Corio Bay, while the hepatic mRNA of TTR and GRP78 were significantly lower in those fish. Positive correlations were observed between Diablo/SMAC1 and CYP1A, D2 and TRβ, TRα and TRβ. This study demonstrates that application of pathway-based biomarker genes and histopathology can provide comprehensive information on the impact of environmental pollutants on fish.

Item Type:	Article
Authors/Creators:	Fu, D and Bridle, A and Leef, M and Gagnon, MM and Hassell, KL and Nowak, BF
Keywords:	biomarkers, histology, gene expression, sand flathead, Port Phillip Bay
Journal or Publication Title:	Marine Pollution Bulletin
Publisher:	Pergamon-Elsevier Science Ltd
ISSN:	0025-326X
DOI / ID Number:	https://doi.org/10.1016/j.marpolbul.2017.03.067
Copyright Information:	© 2017 Elsevier
Related URLs:	Google Scholar: Bridle, A UTAS Profile: Bridle, A UTAS Profile: Leef, M UTAS Profile: Nowak, BF
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