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New strategies for stationary phase integration within centrifugal microfluidic platforms for applications in sample preparation and pre-concentration

Duffy, E, Padovani, R, He, X, Gorkin, R, Vereshchagina, E, Ducree, J, Nesterenko, EP, Nesterenko, PN ORCID: 0000-0002-9997-0650, Brabazon, D, Paull, B ORCID: 0000-0001-6373-6582 and Vazquez, M 2017 , 'New strategies for stationary phase integration within centrifugal microfluidic platforms for applications in sample preparation and pre-concentration' , Analytical Methods, vol. 9, no. 13 , pp. 1998-2006 , doi:

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New approaches for fabrication of centrifugal microfluidic platforms (μCDs) for sample micro-extraction and pre-concentration in bioanalytical and environmental applications are presented. The integration of both octadecylsilica (C18) micro-particulate and porous carbon monolithic stationary phases was demonstrated and on-disc extractions of analytes in samples of different nature were performed. A novel strategy based on the packing of micro-particulate stationary phases using porous organic polymer monoliths as column frits was demonstrated through the in situ photo-polymerisation of monolithic frits in a specific area of the micro-channel, thereby greatly facilitating stationary phase packing within μCD platforms. An enrichment factor of 3.7 was obtained for vitamin B12 following on-disc pre-concentration on the octadecylsilica columns. UV-Vis absorbance measurements were also performed in the outlet reservoir permitting quasi-on-line analysis of the small volume fractions collected after extraction, with limits of detection (LODs) found for vitamin B12 (LOD = 43 μM) being rather similar to those found with a commercially available spectrophotometer (LOD = 37 μM). Furthermore, the first integration of carbon monoliths within microfluidic channels is reported. Carbon monoliths were fabricated as rods and cut into discs for their integration within the microfluidic network, offering a highly porous bimodal structure with low flow-through back-pressures, excellent chemical stability as well as adequate mechanical stability. The carbon monolith-based μCD platform was evaluated as a rapid semi-automated pre-concentration approach suitable for in-field use prior to in-lab HPLC quantitation of pollutants at low concentration levels. Calculated mean recoveries for phenol from tap water spiked-samples by using this on-disc pre-concentration method were 68 ± 4% (n = 4, RSD = 5%).

Item Type: Article
Authors/Creators:Duffy, E and Padovani, R and He, X and Gorkin, R and Vereshchagina, E and Ducree, J and Nesterenko, EP and Nesterenko, PN and Brabazon, D and Paull, B and Vazquez, M
Keywords: centrifugal microfluidic platforms, stationary phase, sample preparation
Journal or Publication Title: Analytical Methods
Publisher: Royal Society of Chemistry
ISSN: 1759-9660
DOI / ID Number:
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Copyright 2017 The Royal Society of Chemistry

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