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Bitter melon protects against ER stress in LS174T colonic epithelial cells

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Kunde, DA ORCID: 0000-0002-6513-032X, Chong, WC, Nerurkar, PV, Ahuja, KDK ORCID: 0000-0002-0323-4692, Just, J ORCID: 0000-0002-7095-8017, Smith, Jason Alfred ORCID: 0000-0001-6313-3298, Guven, N ORCID: 0000-0003-3782-767X and Eri, RD ORCID: 0000-0003-1688-8043 2017 , 'Bitter melon protects against ER stress in LS174T colonic epithelial cells' , Bmc Alternative Medicine, vol. 17 , pp. 1-10 , doi: 10.1186/s12906-016-1522-1.

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Abstract

Background: Bitter Melon (BM) has been used as a functional food in traditional Chinese and Indian medicine formany generations and has gained a great deal of attention due to its apparent benefits in moderating some of thepathogenic processes in a variety of inflammatory conditions. BM extract (BME) has been shown to possess stronganti-oxidant properties. In addition, it can ameliorate oxidative stress and potentially ER stress. There is increasingevidence that oxidative and ER stress are major contributors for intestinal secretory cell dysfunction which leads tolocal inflammation and disease pathogenesis that are hallmarks of inflammatory bowel diseases (IBD). Hence, thesearch for potential therapeutics against ER stress and oxidative stress in intestinal epithelial secretory cells mayprovide valuable resources for the management of IBD. The aim of the present study was to investigate the effectsof BME in ameliorating ER stress in colonic epithelial cells.Methods: Human colonic adenocarcinoma LS174T cells were used for the assessment of BME effects on colonicepithelial cells in vitro. Cell viability was assessed using trypan blue exclusion and the effect of BME in amelioratingtunicamycin (TM)-induced ER stress was determined by analysing the mRNA expression of the common ER stressmarkers; ATF6, XBP1, GRP78, CHOP and PERK by quantitative RT-PCR and GRP78 and CHOP by western blot.Results: In the absence of ER stress, BME exhibited no cell toxicity up to 2.0% w/v and no significant effect on thebasal mRNA expression of ER stress markers in LS174T cells. In contrast, pre-treatment of LS174T cells with BMEfollowed by induction of ER stress resulted in a significant decrease in mRNA expression of ATF6, XBP1, GRP78,CHOP and PERK and protein expression of GRP78 and CHOP. Co-treatment during induction of ER stress and posttreatmentfollowing induction of ER Stress in LS174T cells resulted in a lower but still significant reduction in mRNAexpression levels of most ER stress markers.Conclusions: This is one of the first studies demonstrating the efficacy of BME in reducing expression of ER stressmarkers in colonic epithelial cells suggesting the potential of BME as a dietary intervention in ameliorating ER stressand oxidation in IBD. Interestingly, while the most significant effect was seen with pre-treatment of cells with BMEthere was a reduced but still significant effect when co-treated or even post-treated. This suggests that BME mayeven be effective in modulating ER stress in the face of an existing cell stress environment.

Item Type: Article
Authors/Creators:Kunde, DA and Chong, WC and Nerurkar, PV and Ahuja, KDK and Just, J and Smith, Jason Alfred and Guven, N and Eri, RD
Keywords: Inflammatory bowel disease, Intestinal secretory cells, Endoplasmic reticulum stress, Oxidative stress, Unfolded protein responses, Bitter melon
Journal or Publication Title: Bmc Alternative Medicine
Publisher: BioMed Central
ISSN: 1472-6882
DOI / ID Number: 10.1186/s12906-016-1522-1
Copyright Information:

© The Author(s). 2017 Licensed under Creative Commons Attribution 4.0 International (CC BY 4.0) https://creativecommons.org/licenses/by/4.0/

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