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Comparative performance of four immunological test kits for the detection of Paralytic Shellfish Toxins in Tasmanian shellfish

Dorantes Aranda, JJ ORCID: 0000-0003-1513-6501, Campbell, K, Bradbury, A, Elliott, CT, Harwood, DT, Murray, SA, Ugalde, SC, Wilson, K, Burgoyne, M and Hallegraeff, GM ORCID: 0000-0001-8464-7343 2017 , 'Comparative performance of four immunological test kits for the detection of Paralytic Shellfish Toxins in Tasmanian shellfish' , Toxicon, vol. 125 , pp. 110-119 , doi: 10.1016/j.toxicon.2016.11.262.

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Abstract

Blooms of the toxic dinoflagellate Alexandrium tamarense (Group 1) seriously impacted the Tasmanian shellfish industry during 2012 and 2015, necessitating product recalls and intensive paralytic shellfish toxin (PST) product testing. The performance of four commercial PST test kits, Abraxis™, Europroxima™, Scotia™ and Neogen™, was compared with the official AOAC LC-FLD method for contaminated mussels and oysters. Abraxis and Europroxima kits underestimated PST in 35–100% of samples when using standard protocols but quantification improved when concentrated extracts were further diluted (underestimation ≤18%). The Scotia kit (cut off 0.2–0.7 mg STX-diHCl eq/kg) delivered 0% false negatives, but 27% false positives. Neogen produced 5% false negatives and 13% false positives when the cut off was altered to 0.5–0.6 mg STX-diHCl eq/kg, the introduction of a conversion step eliminated false negatives. Based on their sensitivity, ease of use and performance, the Neogen kit proved the most suitable kit for use with Tasmanian mussels and oysters. Once formally validated for regulatory purposes, the Neogen kit could provide shellfish growers with a rapid tool for harvesting decisions at the farm gate. Effective rapid screening preventing compliant samples undergoing testing using the more expensive and time consuming LC-FLD method will result in significant savings in analytical costs.

Item Type: Article
Authors/Creators:Dorantes Aranda, JJ and Campbell, K and Bradbury, A and Elliott, CT and Harwood, DT and Murray, SA and Ugalde, SC and Wilson, K and Burgoyne, M and Hallegraeff, GM
Keywords: Paralytic shellfish poisoning, mussels, oysters, Alexandrium tamarense, paralytic shellfish toxins, immunological test kits, Gonyautotoxin 1&4
Journal or Publication Title: Toxicon
Publisher: Pergamon-Elsevier Science Ltd
ISSN: 0041-0101
DOI / ID Number: 10.1016/j.toxicon.2016.11.262
Copyright Information:

Copyright 2016 Elsevier Ltd.

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