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Optical coherence tomographybased contactindentation for diaphragm mechanics in a mouse model of transforming growth factor alpha induced lung disease

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Wang, KC, Astell, CJ, Wijesinghe, P, Larcombe, AN, Pinniger, GJ, Zosky, GR ORCID: 0000-0001-9039-0302, Kennedy, BF, Berry, LJ, Sampson, DD, James, AL, Le Cras, TD and Noble, PB 2017 , 'Optical coherence tomographybased contactindentation for diaphragm mechanics in a mouse model of transforming growth factor alpha induced lung disease' , Scientific reports, vol. 7 , pp. 1-10 , doi: 10.1038/s41598-017-01431-x.

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Abstract

This study tested the utility of optical coherence tomography (OCT)-based indentation to assessmechanical properties of respiratory tissues in disease. Using OCT-based indentation, the elasticmodulus of mouse diaphragm was measured from changes in diaphragm thickness in response toan applied force provided by an indenter. We used a transgenic mouse model of chronic lung diseaseinduced by the overexpression of transforming growth factor-alpha (TGF-α), established by thepresence of pleural and peribronchial fibrosis and impaired lung mechanics determined by the forcedoscillation technique and plethysmography. Diaphragm elastic modulus assessed by OCT-basedindentation was reduced by TGF-α at both left and right lateral locations (p<0.05). Diaphragm elasticmodulus at left and right lateral locations were correlated within mice (r=0.67, p<0.01) suggestingthat measurements were representative of tissue beyond the indenter field. Co-localised images ofdiaphragm after TGF-α overexpression revealed a layered fibrotic appearance. Maximum diaphragmforce in conventional organ bath studies was also reduced by TGF-α overexpression (p<0.01). Resultsshow that OCT-based indentation provided clear delineation of diseased diaphragm, and togetherwith organ bath assessment, provides new evidence suggesting that TGF-α overexpression producesimpairment in diaphragm function and, therefore, an increase in the work of breathing in chronic lungdisease.

Item Type: Article
Authors/Creators:Wang, KC and Astell, CJ and Wijesinghe, P and Larcombe, AN and Pinniger, GJ and Zosky, GR and Kennedy, BF and Berry, LJ and Sampson, DD and James, AL and Le Cras, TD and Noble, PB
Keywords: TGF-a, optical coherence tomography, diaphragm function
Journal or Publication Title: Scientific reports
Publisher: Nature Publishing Group
ISSN: 2045-2322
DOI / ID Number: 10.1038/s41598-017-01431-x
Copyright Information:

Copyright 2017 The Authors. Licensed under Creative Commons Attribution 4.0 International (CC BY 4.0) https://creativecommons.org/licenses/by/4.0/

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