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Y-linked iDmrt1 paralogue (iDMY) in the Eastern spiny lobster, Sagmariasus verreauxi: the first invertebrate sex-linked Dmrt

Chandler, JC, Fitzgibbon, QP ORCID: 0000-0002-1104-3052, Smith, G ORCID: 0000-0002-8677-1230, Elizur, A and Ventura, T 2017 , 'Y-linked iDmrt1 paralogue (iDMY) in the Eastern spiny lobster, Sagmariasus verreauxi: the first invertebrate sex-linked Dmrt' , Developmental Biology, vol. 430, no. 2 , pp. 337-345 , doi: 10.1016/j.ydbio.2017.08.031.

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Abstract

Sex determination pathways are extensively diverse across species, with the master sex-determinants being the most variable element. Despite this, there is a family of DM-domain transcription factors (Dmrts), which hold a highly conserved function in sexual development. This work is the first to describe a heterogametic sex-linked Dmrt in an invertebrate species, the Eastern spiny lobster, Sagmariasus verreauxi. We have termed the Ylinked, truncated paralogue of the autosomal iDmrt1, Sv-iDMY. Considering the master sex-determining function of both DMY in medaka and DM-W in frog, we hypothesised a similar function of Sv-iDMY. By conducting temporal expression analyses during embryogenesis we have identified a putative male sexdetermining period during which iDMY > iDmrt1. Employing a GAL4-transactivation assay we then demonstrate the dominant negative suppression of iDMY over its autosomal iDmrt1 paralogue, suggesting the mechanism with which iDMY determines sex. Comparative analyses of Sv-iDMY, DM-W and medaka DMY, highlight the C′-mediated features of oligomerisation and transactivation as central to the mechanism that each exerts. Indeed, these features may underpin the plasticity facilitating the convergent emergence of these three sporadic sex-linked master-Dmrts.

Item Type: Article
Authors/Creators:Chandler, JC and Fitzgibbon, QP and Smith, G and Elizur, A and Ventura, T
Keywords: master sex-determinant, Dmrt, DMY, transactivation domain, Crustacea
Journal or Publication Title: Developmental Biology
Publisher: Academic Press Inc Elsevier Science
ISSN: 0012-1606
DOI / ID Number: 10.1016/j.ydbio.2017.08.031
Copyright Information:

Copyright 2017 Elsevier Inc.

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